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Cell Biology Proteolysis / Ubiquitin Proteolytic enzymes Metalloprotease ACEs
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Mouse ACE2 ELISA Kit (ab213843)

  • Datasheet
  • SDS
  • Protocol Booklet
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Mouse ACE2 ELISA Kit (ab213843) Standard Curve.

    Key features and details

    • Sensitivity: 10 pg/ml
    • Range: 62.5 pg/ml - 4000 pg/ml
    • Sample type: Cell culture supernatant, Hep Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Mouse

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    Product image
    Human ACE2 ELISA Kit (ab235649)

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    Overview

    • Product name

      Mouse ACE2 ELISA Kit
      See all ACE2 kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      1 16 146pg/ml 11.38 7.8%
      2 16 538pg/ml 27.88 4.7%
      3 16 1252pg/ml 75.77 6.1%
      Inter-assay
      Sample n Mean SD CV%
      1 24 131pg/ml 10.48 8%
      2 24 557pg/ml 31.74 5.7%
      3 24 1192pg/ml 84.63 7.1%
    • Sample type

      Cell culture supernatant, Serum, Hep Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      < 10 pg/ml
    • Range

      62.5 pg/ml - 4000 pg/ml
    • Assay time

      3h 30m
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Mouse
    • Product overview

      The Mouse ACE2 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213843) is designed for the quantitative measurement of Mouse ACE2 in cell culture supernatants, serum and plasma (heparin).


      The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for ACE2 has been pre-coated onto 96-well plates. Standards (Expression system for standard: CHO; Immunogen sequence: Q18-T740) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ACE2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse ACE2 amount of sample captured in plate.

    • Notes

      Angiotensin-converting enzyme 2 (ACE 2) is a protein belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. By sequence similarity to a sequence in GenBank, this gene is mapped to Xp22.2. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.

    • Platform

      Pre-coated microplate (12 x 8 well strips)

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components Identifier 1 x 96 tests
      ABC Diluent Buffer Blue Cap 1 x 12ml
      Adhesive Plate Seal 4 units
      Antibody Diluent Buffer Green Cap 1 x 12ml
      Anti-Mouse ACE2 coated Microplate (12 x 8 wells) 1 unit
      Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl
      Biotinylated anti- Mouse ACE2 antibody 1 x 130µl
      Lyophilized recombinant Mouse ACE2 standard 2 vials
      Sample Diluent Buffer Green Cap 1 x 30ml
      TMB Color Developing Agent Black Cap 1 x 10ml
      TMB Stop Solution Yellow Cap 1 x 10ml
    • Research areas

      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteolytic enzymes
      • Metalloprotease
      • ACEs
      • Cardiovascular
      • Blood
      • Blood Pressure regulation
      • Cardiovascular
      • Heart
      • Contractility
      • Inotropics
      • Cardiovascular
      • Vasculature
      • Vasoconstriction
      • Other
    • Function

      Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
    • Tissue specificity

      Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.
    • Sequence similarities

      Belongs to the peptidase M2 family.
    • Post-translational
      modifications

      N-glycosylation on Asn-90 may limit SARS infectivity.
    • Cellular localization

      Secreted and Cell membrane.
    • Target information above from: UniProt accession Q9BYF1 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • ACE 2
      • ACE related carboxypeptidase
      • ACE-related carboxypeptidase
      • ACE2
      • ACE2_HUMAN
      • ACEH
      • Angiotensin converting enzyme 2
      • Angiotensin converting enzyme homolog
      • Angiotensin converting enzyme like protein
      • Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2
      • Angiotensin I converting enzyme 2
      • Angiotensin-converting enzyme homolog
      • DKFZP434A014
      • EC 3.4.17
      • metalloprotease MPROT 15
      • Metalloprotease MPROT15
      • OTTHUMP00000022963
      • Processed angiotensin-converting enzyme 2
      see all
    • Database links

      • Entrez Gene: 70008 Mouse
      • SwissProt: Q8R0I0 Mouse
      • Unigene: 13451 Mouse

      Associated products

        Images

        • Mouse ACE2 ELISA Kit (ab213843) Standard Curve.
          Mouse ACE2 ELISA Kit (ab213843) Standard Curve.

          Mouse ACE2 ELISA Kit (ab213843) Standard Curve.

        Protocols

        • Protocol Booklet

        Click here to view the general protocols

        Datasheets and documents

        • Datasheet
        • SDS
      • References (4)

        Publishing research using ab213843? Please let us know so that we can cite the reference in this datasheet.

        ab213843 has been referenced in 4 publications.

        • Evans CE  et al. ACE2 activation protects against cognitive decline and reduces amyloid pathology in the Tg2576 mouse model of Alzheimer's disease. Acta Neuropathol 139:485-502 (2020). PubMed: 31982938
        • Han W  et al. Chemical renal denervation-induced upregulation of the ACE2/Ang (1-7)/Mas axis attenuates blood pressure elevation in spontaneously hypertensive rats. Clin Exp Hypertens 42:661-668 (2020). PubMed: 32476477
        • Pan X  et al. FGF21 Prevents Angiotensin II-Induced Hypertension and Vascular Dysfunction by Activation of ACE2/Angiotensin-(1-7) Axis in Mice. Cell Metab 27:1323-1337.e5 (2018). PubMed: 29706566
        • Zheng J  et al. Effects of IQP, VEP and Spirulina platensis hydrolysates on the local kidney renin angiotensin system in spontaneously hypertensive rats. Mol Med Rep 16:8485-8492 (2017). PubMed: 28944898

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