Overview

  • Product name
    Mouse AIF Antibody Pair - BSA and Azide free
    See all AIF kits
  • Detection method
    Colorimetric
  • Assay type
    ELISA set
  • Range
    7.81 ng/ml - 500 ng/ml
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Product overview

    Antibody Pairs – BSA and Azide free include a capture and a detector antibody pair suitable for sandwich ELISA. The Antibody Pair can be used to quantify native and recombinant Mouse AIF.


    For additional information on the performance of the antibody pair used in this kit, please see equivalent SimpleStep ELISA® Kit (ab235651), which use the same antibody pair. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    Please note that the range provided is only an estimation based on the performance of a related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay.


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.


    Download SDS here.


     

  • Tested applications
    Suitable for: ELISAmore details
  • Platform
    Reagents

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 10 x 96 tests
    Mouse AIF Capture Antibody 1 x 100µg
    Mouse AIF Detector Antibody 1 x 100µg
  • Research areas
  • Function
    Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
  • Involvement in disease
    Defects in AIFM1 are the cause of combined oxidative phosphorylation deficiency type 6 (COXPD6) [MIM:300816]. It is a mitochondrial disease resulting in a neurodegenerative disorder characterized by psychomotor delay, hypotonia, areflexia, muscle weakness and wasting.
  • Sequence similarities
    Belongs to the FAD-dependent oxidoreductase family.
  • Post-translational
    modifications
    Under normal conditions, a 54-residue N-terminal segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase (MPP) to form the inner-membrane-anchored mature form (AIFmit). During apoptosis, it is further proteolytically processed at amino-acid position 101 leading to the generation of the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner.
  • Cellular localization
    Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region.
  • Information by UniProt
  • Alternative names
    • AIFM1
    • AIFM1_HUMAN
    • Apoptosis inducing factor
    • Apoptosis inducing factor 1, mitochondrial
    • Apoptosis inducing factor, mitochondrion associated, 1
    • Apoptosis-inducing factor 1
    • CMTX4
    • COWCK
    • COXPD6
    • Harlequin
    • Hq
    • mAIF
    • MGC111425
    • MGC5706
    • mitochondrial
    • Neuropathy, axonal motor-sensory, with deafness and mental retardation
    • neuropathy, axonal, motor-sensory with deafness and mental retardation (Cowchock syndrome)
    • PDCD 8
    • PDCD8
    • Programmed cell death 8
    • Programmed cell death 8 (apoptosis inducing factor)
    • Programmed cell death 8 isoform 1
    • Programmed cell death 8 isoform 2
    • Programmed cell death 8 isoform 3
    • Programmed cell death protein 8
    • Programmed cell death protein 8 mitochondrial
    • Programmed cell death protein 8 mitochondrial precursor
    • Striatal apoptosis inducing factor
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab244137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

References

ab244137 has not yet been referenced specifically in any publications.

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