Mouse AKT2 Matched Antibody Pair Kit (ab216054)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 62.5 pg/ml - 4000 pg/ml
Overview
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Product name
Mouse AKT2 Matched Antibody Pair Kit
See all AKT2 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
10.7 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse AKT2 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse AKT2.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab208986. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse AKT2 Capture Antibody 2 x 50µg 1 x 50µg Mouse AKT2 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse AKT2 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
General protein kinase capable of phosphorylating several known proteins. -
Tissue specificity
Expressed in all human cell types so far analyzed. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylation on Thr-309 and Ser-474 is required for full activity.
Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. - Information by UniProt
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Alternative names
- Akt2
- AKT2_HUMAN
- HIHGHH
see all -
Database links
- Entrez Gene: 11652 Mouse
- SwissProt: Q60823 Mouse
- Unigene: 177194 Mouse
Associated products
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Alternative Versions
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Related Products
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SimpleStep ELISA kits
Applications
Our Abpromise guarantee covers the use of ab216054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. |
Images
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Mouse AKT2 standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse AKT2 Matched Antibody Pair Kit (ab216054)To learn more about the advantages of recombinant antibodies see here.
References (0)
ab216054 has not yet been referenced specifically in any publications.
