Mouse AKT2 Matched Antibody Pair Kit (ab216054)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameMouse AKT2 Matched Antibody Pair Kit
See all AKT2 kits -
Detection methodColorimetric
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Assay typeELISA set
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Range62.5 pg/ml - 4000 pg/ml
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Species reactivityReacts with: Mouse
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Product overview
Matched Antibody Pair kit is a titrated unlabeled capture antibody, a titrated biotin-labeled detector and a calibrated protein standard. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse AKT2.
Both capture and detector antibodies are rabbit monoclonal antibodies.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Additional protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website.
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab208986) which uses the same antibody pair.
To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.
Buffer information:
The capture antibody is glycerol free. The detector antibody contains glycerol. -
Tested applicationsSuitable for: ELISAmore details
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PlatformReagents
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 10 x 96 tests 2 x 96 tests Mouse AKT2 Capture Antibody 1 x 100µg 1 x 20µg Mouse AKT2 Detector Antibody 1 x 25µg 1 x 5µg Mouse AKT2 Lyophilized Protein 1 vial 1 vial -
Research areas
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FunctionGeneral protein kinase capable of phosphorylating several known proteins.
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Tissue specificityExpressed in all human cell types so far analyzed.
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Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylation on Thr-309 and Ser-474 is required for full activity.
Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. - Information by UniProt
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Alternative names
- Akt2
- AKT2_HUMAN
- HIHGHH
see all -
Database links
- Entrez Gene: 11652 Mouse
- SwissProt: Q60823 Mouse
- Unigene: 177194 Mouse
Associated products
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SimpleStep ELISA kits
Applications
Our Abpromise guarantee covers the use of ab216054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. |
Images
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Mouse AKT2 standard curve.Standard calibration curve. Background subtracted values are graphed.
Protocols
References
ab216054 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"