Mouse AKT2 Matched Antibody Pair Kit (ab216054)
- Datasheet
- References
- Protocols
Overview
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Product name
Mouse AKT2 Matched Antibody Pair Kit
See all AKT2 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
10.7 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse AKT2 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse AKT2.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab208986. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
We’ve listened to you: due to popular demand, we will now provide our Matched Antibody Pair kits in 5x96 tests and 10x96 tests. The 2x96 tests size will be discontinued on 30th June 2020 unless inventory is depleted beforehand.
To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse AKT2 Capture Antibody 1 x 100µg 1 x 50µg Mouse AKT2 Detector Antibody 1 x 25µg 1 x 12.5µg Mouse AKT2 Lyophilized Protein 1 vial 1 vial -
Research areas
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Function
General protein kinase capable of phosphorylating several known proteins. -
Tissue specificity
Expressed in all human cell types so far analyzed. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylation on Thr-309 and Ser-474 is required for full activity.
Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. - Information by UniProt
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Alternative names
- Akt2
- AKT2_HUMAN
- HIHGHH
see all -
Database links
- Entrez Gene: 11652 Mouse
- SwissProt: Q60823 Mouse
- Unigene: 177194 Mouse
Associated products
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Alternative Versions
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SimpleStep ELISA kits
Applications
Our Abpromise guarantee covers the use of ab216054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. |
Images
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Mouse AKT2 standard curve.Standard calibration curve. Background subtracted values are graphed.
Protocols
References
ab216054 has not yet been referenced specifically in any publications.
