Overview

  • Product name

    Mouse Albumin ELISA Kit
    See all Albumin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5.4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.3%
  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 0.62 ng/ml
  • Range

    0.781 ng/ml - 200 ng/ml
  • Recovery

    97 %

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Mouse Albumin ELISA kit is designed for the quantitative measurement of mouse Albumin levels in urine and cell culture supernatants.


    An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Albumin captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve Using ab108792.

Protocols

References

This product has been referenced in:

  • Tong S  et al. Role of neutrophil extracellular traps in chronic kidney injury induced by bisphenol-A. J Endocrinol N/A:N/A (2019). Read more (PubMed: 30798321) »
  • Jiang ZJ  et al. Galectin-1 gene silencing inhibits the activation and proliferation but induces the apoptosis of hepatic stellate cells from mice with liver fibrosis. Int J Mol Med 43:103-116 (2019). Read more (PubMed: 30365068) »
See all 27 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer


ab108792: We have never tested brain tissue supernatants so I do not know if it would work. I cannot recommend the dilution range.

ab119557: Again, we have not tested brain tissue with this kit but there is a publication where a FlowCytomix kit, which uses the same antibodies, was used for brain tissues. Therefore, I would assume that this kit would work on your samples however, I cannot guarantee this.

The publication is the following and may be of interest to you:

Nogo-Receptors NgR1 and NgR2 Do Not Mediate Regulation of CD4 T Helper Responses and CNS Repair in Experimental Autoimmune Encephalomyelitis, Karin Steinbach et al, http://www.plosone.org 8 November 2011 | Volume 6 | Issue 11 | e26341

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Answer

Thank you for your enquiry. I am sorry to hear the customer has some concerns regarding this kit, I hope I can be of help. For the standard,  as described in the protocol this needs to be reconstituted with the appropriate amount of diluent to generate a stock solution of 400 ng/ml.   If the customer has 360 ng of standard in their vial, they can calculate how much diluent to reconstitute this in to obtain a concentration of 400 ng/ml: 400 ng/ml divided by 360 ng/ml = 1.1 To work out the dilution factor, divide 1 ml by 1.1 = 0.909ml of diluent Therefore, dissolve the 360 ng in 909 ul of diluent, and this will give a concentration of 400 mg/ml. This can be used as the stock solution. Although the standard curve shown with the protocol goes up to 800 ng/ml, I think the protocol does not mention to use this concentration, because the graph is starting to plateau at this point, and is out of the linear range of the assay.  therefore, data collected at this higher concentration would not be accurate. For the wash buffer and diluent, I can suggest to mix them thoroughly, use a vortex mixer if necessary. Leave them at room temperature for half an hour to warm up the vial and help the precipitate to dissolve. I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

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