Mouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready To Use) (ab93697)

Overview

  • Product name

    Mouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready To Use)
    See all Polyvalent HRP kits
  • Product overview

    The reagents in this kit constitute a labeled streptavidin-biotin immunoenzymatic antigen detection system with increased sensitivity. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.


    Reacts with Mouse and Rabbit IgG (H+L)

  • Tested applications

    Suitable for: IHC-Pmore details

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 60 ml 125 ml 60 ml 125 ml
    Biotinylated Goat Anti-Polyvalent Plus 1 x 60ml 1 x 125ml 1 x 60ml 1 x 125ml
    Protein block 1 x 60ml 1 x 125ml 1 x 60ml 1 x 125ml
    Streptavidin Peroxidase Plus 1 x 60ml 1 x 125ml 1 x 60ml 1 x 125ml
  • Research areas

  • Relevance

    Polyvalent HRP is a labeled streptavidin-biotin immunoenzymatic antigen detection system. Horseradish peroxidase (HRP) readily combines with hydrogen peroxide (H2O2) and the resultant complex can oxidize a wide variety of chromogenic substrates.
  • Alternative names

    • Polyvalent/HRP

Applications

Our Abpromise guarantee covers the use of ab93697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

Images

  • Immunohistochemistry analysis of mouse femur tissue sections. Femurs were fixed in 10% neutral buffered formalin and decalcified for 7 days in 15% EDTA; complete decalcification was confirmed by X-ray. Decalcified bones were embedded in paraffin and sectioned longitudinally through the defect at a thickness of 8 microns. Immunohistochemical staining was performed with monoclonal anti-activated beta-catenin (un-phosphorylated on Ser33/37 and Thr41), or an IgG isotype control. Chromogens were developed using ab93697, Mouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready to Use), followed by 3′-3′-diaminobenzidine (DAB), and then counterstained with fast green. Beta-catenin levels are elevated in defects of Sost-/- mice.

Protocols

References

This product has been referenced in:

  • Aktas S  et al. Evaluation of the Rho A/Rho-kinase pathway in the uterus of the rat model of polycystic ovary syndrome. Reprod Biol 19:45-54 (2019). Read more (PubMed: 30704840) »
  • Sanaii A  et al. Role of Th22 cells in Helicobacter pylori-related gastritis and peptic ulcer diseases. Mol Biol Rep N/A:N/A (2019). Read more (PubMed: 31359381) »
See all 6 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your enquiry on the phone this morning.

Following on from our discussions, I can recommend to contact the supplier of the antibody that is not providinga strong enough signal in your experiments as it is possible there is a problem with the antibody, or they may be able to provide some more product specific advice. Also, if you colleague that you obtained this from is in a different place and hassent this to you by courier, it may be that it has been damaged by shipping and itmay be better to try a freshvial.

If you would still like to try amplification of the signal, I can confirm that we have the following 2 amplification detection kits which utilize a streptavidin complex:

ab93697 Mouse and Rabbit specific HRP/DAB Plus detection IHC kit
https://www.abcam.com/index.html?datasheet=93697 (or use the following: https://www.abcam.com/index.html?datasheet=93697).

ab94698 Mouse and Rabbit specific HRP/DAB Plus detection IHC kit
https://www.abcam.com/index.html?datasheet=94698 (or use the following: https://www.abcam.com/index.html?datasheet=94698).

I suggest you could try the following fluorescent conjugated streptavidin antibodies with this for detection (rather than the HRP). However I am sorry they are not testedfor this type ofapplication:

ab7240 Anti-Streptavidin antibody (Texas Red ®)
Rabbit polyclonal
Texas Red conjugated
https://www.abcam.com/index.html?datasheet=7240 (or use the following: https://www.abcam.com/index.html?datasheet=7240).

ab7238 Anti-Streptavidin antibody (FITC)
Rabbit polyclonal
FITC conjugated Applications
https://www.abcam.com/index.html?datasheet=7238 (or use the following: https://www.abcam.com/index.html?datasheet=7238).

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contactus.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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