Mouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready To Use) (ab93697)
Overview
-
Product name
Mouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready To Use)
See all Polyvalent HRP kits -
Product overview
The reagents in this kit constitute a labeled streptavidin-biotin immunoenzymatic antigen detection system with increased sensitivity. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.
Reacts with Mouse and Rabbit IgG (H+L)
-
Tested applications
Suitable for: IHC-Pmore details
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 60 ml 125 ml Biotinylated Goat Anti-Polyvalent Plus 1 x 60ml 1 x 125ml Protein block 1 x 60ml 1 x 125ml Streptavidin Peroxidase Plus 1 x 60ml 1 x 125ml -
Research areas
-
Relevance
Polyvalent HRP is a labeled streptavidin-biotin immunoenzymatic antigen detection system. Horseradish peroxidase (HRP) readily combines with hydrogen peroxide (H2O2) and the resultant complex can oxidize a wide variety of chromogenic substrates. -
Alternative names
- Polyvalent/HRP
Associated products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent dilution.
|
| Notes |
|---|
|
IHC-P
Use at an assay dependent dilution. |
Images
-
Mouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready To Use)Image from McGee-Lawrence ME et al., Biochem Biophys Res Commun. 2013;411(4):886-90. Fig 3.; doi: 10.1016/j.bbrc.2013.10.155 with permission from Elsevier.
Immunohistochemistry analysis of mouse femur tissue sections. Femurs were fixed in 10% neutral buffered formalin and decalcified for 7 days in 15% EDTA; complete decalcification was confirmed by X-ray. Decalcified bones were embedded in paraffin and sectioned longitudinally through the defect at a thickness of 8 microns. Immunohistochemical staining was performed with monoclonal anti-activated beta-catenin (un-phosphorylated on Ser33/37 and Thr41), or an IgG isotype control. Chromogens were developed using ab93697, Mouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready to Use), followed by 3′-3′-diaminobenzidine (DAB), and then counterstained with fast green. Beta-catenin levels are elevated in defects of Sost-/- mice.
References (6)
ab93697 has been referenced in 6 publications.
- Aktas S et al. Evaluation of the Rho A/Rho-kinase pathway in the uterus of the rat model of polycystic ovary syndrome. Reprod Biol 19:45-54 (2019). PubMed: 30704840
- Sanaii A et al. Role of Th22 cells in Helicobacter pylori-related gastritis and peptic ulcer diseases. Mol Biol Rep N/A:N/A (2019). PubMed: 31359381
- Sabzevary-Ghahfarokhi M et al. The regulatory role of Nrf2 in antioxidants phase2 enzymes and IL-17A expression in patients with ulcerative colitis. Pathol Res Pract 214:1149-1155 (2018). PubMed: 29941222
- Al Asmari AK & Khan AQ Investigation of in vivo potential of scorpion venom against skin tumorigenesis in mice via targeting markers associated with cancer development. Drug Des Devel Ther 10:3387-3397 (2016). IHC ; Mouse . PubMed: 27799739
- Carpio LR et al. Histone deacetylase 3 supports endochondral bone formation by controlling cytokine signaling and matrix remodeling. Sci Signal 9:ra79 (2016). IHC-P ; Mouse . PubMed: 27507649
- McGee-Lawrence ME et al. Sclerostin deficient mice rapidly heal bone defects by activating ß-catenin and increasing intramembranous ossification. Biochem Biophys Res Commun 441:886-90 (2013). IHC-P . PubMed: 24211207
