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Mouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready To Use) (ab93697)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (1)References (6)

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Mouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready To Use)

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    Overview

    • Product name

      Mouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready To Use)
      See all Polyvalent HRP kits
    • Product overview

      The reagents in this kit constitute a labeled streptavidin-biotin immunoenzymatic antigen detection system with increased sensitivity. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.


      Reacts with Mouse and Rabbit IgG (H+L)

    • Tested applications

      Suitable for: IHC-Pmore details

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 60 ml 125 ml
      Biotinylated Goat Anti-Polyvalent Plus 1 x 60ml 1 x 125ml
      Protein block 1 x 60ml 1 x 125ml
      Streptavidin Peroxidase Plus 1 x 60ml 1 x 125ml
    • Research areas

      • Kits/ Lysates/ Other
      • Kits
      • IHC Kits
      • Kits/ Lysates/ Other
      • Kits
      • IHC tools
      • ABC
    • Relevance

      Polyvalent HRP is a labeled streptavidin-biotin immunoenzymatic antigen detection system. Horseradish peroxidase (HRP) readily combines with hydrogen peroxide (H2O2) and the resultant complex can oxidize a wide variety of chromogenic substrates.
    • Alternative names

      • Polyvalent/HRP

    Associated products

      Applications

      The Abpromise guarantee

      Our Abpromise guarantee covers the use of ab93697 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Abreviews Notes
      IHC-P
      Use at an assay dependent dilution.
      Notes
      IHC-P
      Use at an assay dependent dilution.

      Images

      • Mouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready To Use)
        Mouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready To Use)Image from McGee-Lawrence ME et al., Biochem Biophys Res Commun. 2013;411(4):886-90. Fig 3.; doi: 10.1016/j.bbrc.2013.10.155 with permission from Elsevier.

        Immunohistochemistry analysis of mouse femur tissue sections. Femurs were fixed in 10% neutral buffered formalin and decalcified for 7 days in 15% EDTA; complete decalcification was confirmed by X-ray. Decalcified bones were embedded in paraffin and sectioned longitudinally through the defect at a thickness of 8 microns. Immunohistochemical staining was performed with monoclonal anti-activated beta-catenin (un-phosphorylated on Ser33/37 and Thr41), or an IgG isotype control. Chromogens were developed using ab93697, Mouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready to Use), followed by 3′-3′-diaminobenzidine (DAB), and then counterstained with fast green. Beta-catenin levels are elevated in defects of Sost-/- mice.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (6)

      Publishing research using ab93697? Please let us know so that we can cite the reference in this datasheet.

      ab93697 has been referenced in 6 publications.

      • Aktas S  et al. Evaluation of the Rho A/Rho-kinase pathway in the uterus of the rat model of polycystic ovary syndrome. Reprod Biol 19:45-54 (2019). PubMed: 30704840
      • Sanaii A  et al. Role of Th22 cells in Helicobacter pylori-related gastritis and peptic ulcer diseases. Mol Biol Rep N/A:N/A (2019). PubMed: 31359381
      • Sabzevary-Ghahfarokhi M  et al. The regulatory role of Nrf2 in antioxidants phase2 enzymes and IL-17A expression in patients with ulcerative colitis. Pathol Res Pract 214:1149-1155 (2018). PubMed: 29941222
      • Al Asmari AK & Khan AQ Investigation of in vivo potential of scorpion venom against skin tumorigenesis in mice via targeting markers associated with cancer development. Drug Des Devel Ther 10:3387-3397 (2016). IHC ; Mouse . PubMed: 27799739
      • Carpio LR  et al. Histone deacetylase 3 supports endochondral bone formation by controlling cytokine signaling and matrix remodeling. Sci Signal 9:ra79 (2016). IHC-P ; Mouse . PubMed: 27507649
      • McGee-Lawrence ME  et al. Sclerostin deficient mice rapidly heal bone defects by activating ß-catenin and increasing intramembranous ossification. Biochem Biophys Res Commun 441:886-90 (2013). IHC-P . PubMed: 24211207

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a question

      Question

      We have an antibody (a non Abcam one) that is providing a weak signal.
      Do you have a fluorescent amplification kit?
      Or one with streptavidin that we could then use a fluorescent conjugated anti- streptavidin antibody on?

      Read More

      Abcam community

      Verified customer

      Asked on Jan 20 2012

      Answer

      Thank you for your enquiry on the phone this morning.

      Following on from our discussions, I can recommend to contact the supplier of the antibody that is not providinga strong enough signal in your experiments as it is possible there is a problem with the antibody, or they may be able to provide some more product specific advice. Also, if you colleague that you obtained this from is in a different place and hassent this to you by courier, it may be that it has been damaged by shipping and itmay be better to try a freshvial.

      If you would still like to try amplification of the signal, I can confirm that we have the following 2 amplification detection kits which utilize a streptavidin complex:

      ab93697 Mouse and Rabbit specific HRP/DAB Plus detection IHC kit
      https://www.abcam.com/Mouse-and-Rabbit-Specific-HRP-Plus-ABC-Detection-IHC-Kit-Ready-To-Use-ab93697.html (or use the following: https://www.abcam.com/Mouse-and-Rabbit-Specific-HRP-Plus-ABC-Detection-IHC-Kit-Ready-To-Use-ab93697.html).

      ab94698 Mouse and Rabbit specific HRP/DAB Plus detection IHC kit
      https://www.abcam.com/Mouse-and-Rabbit-Specific-HRP-Plus-ABC-Detection-IHC-Kit-ab94698.html (or use the following: https://www.abcam.com/Mouse-and-Rabbit-Specific-HRP-Plus-ABC-Detection-IHC-Kit-ab94698.html).

      I suggest you could try the following fluorescent conjugated streptavidin antibodies with this for detection (rather than the HRP). However I am sorry they are not testedfor this type ofapplication:

      ab7240 Anti-Streptavidin antibody (Texas Red ®)
      Rabbit polyclonal
      Texas Red conjugated
      https://www.abcam.com/Streptavidin-antibody-Texas-Red--ab7240.html (or use the following: https://www.abcam.com/Streptavidin-antibody-Texas-Red--ab7240.html).

      ab7238 Anti-Streptavidin antibody (FITC)
      Rabbit polyclonal
      FITC conjugated Applications
      https://www.abcam.com/Streptavidin-antibody-FITC-ab7238.html (or use the following: https://www.abcam.com/Streptavidin-antibody-FITC-ab7238.html).

      I hope this will be helpful to you. If you have any further questions, please do not hesitate to contactus.

      Read More

      Abcam Scientific Support

      Answered on Jan 20 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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