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We have an antibody (a non Abcam one) that is providing a weak signal.
Do you have a fluorescent amplification kit?
Or one with streptavidin that we could then use a fluorescent conjugated anti- streptavidin antibody on?
Asked on Jan 20 2012
Thank you for your enquiry on the phone this morning.
Following on from our discussions, I can recommend to contact the supplier of the antibody that is not providinga strong enough signal in your experiments as it is possible there is a problem with the antibody, or they may be able to provide some more product specific advice. Also, if you colleague that you obtained this from is in a different place and hassent this to you by courier, it may be that it has been damaged by shipping and itmay be better to try a freshvial.
If you would still like to try amplification of the signal, I can confirm that we have the following 2 amplification detection kits which utilize a streptavidin complex:
ab93697 Mouse and Rabbit specific HRP/DAB Plus detection IHC kit
https://www.abcam.com/Mouse-and-Rabbit-Specific-HRP-Plus-ABC-Detection-IHC-Kit-Ready-To-Use-ab93697.html (or use the following: https://www.abcam.com/Mouse-and-Rabbit-Specific-HRP-Plus-ABC-Detection-IHC-Kit-Ready-To-Use-ab93697.html).
ab94698 Mouse and Rabbit specific HRP/DAB Plus detection IHC kit
https://www.abcam.com/Mouse-and-Rabbit-Specific-HRP-Plus-ABC-Detection-IHC-Kit-ab94698.html (or use the following: https://www.abcam.com/Mouse-and-Rabbit-Specific-HRP-Plus-ABC-Detection-IHC-Kit-ab94698.html).
I suggest you could try the following fluorescent conjugated streptavidin antibodies with this for detection (rather than the HRP). However I am sorry they are not testedfor this type ofapplication:
ab7240 Anti-Streptavidin antibody (Texas Red ®)
Texas Red conjugated
https://www.abcam.com/Streptavidin-antibody-Texas-Red--ab7240.html (or use the following: https://www.abcam.com/Streptavidin-antibody-Texas-Red--ab7240.html).
ab7238 Anti-Streptavidin antibody (FITC)
FITC conjugated Applications
https://www.abcam.com/Streptavidin-antibody-FITC-ab7238.html (or use the following: https://www.abcam.com/Streptavidin-antibody-FITC-ab7238.html).
I hope this will be helpful to you. If you have any further questions, please do not hesitate to contactus.
Answered on Jan 20 2012