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    mouse-and-rabbit-specific-hrpaec-abc-detection-ihc-kit-ab93705.pdf

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Mouse and Rabbit Specific HRP/AEC (ABC) Detection IHC Kit (ab93705)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (1)Q&A (1)References (11)

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CD34 detection using HRP/AEC Detection Kit (ab93705)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/AEC detection IHC kit  (ab93705)

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Overview

  • Product name

    Mouse and Rabbit Specific HRP/AEC (ABC) Detection IHC Kit
    See all HRP/AEC Detection Kit kits
  • Product overview

    Mouse and Rabbit specific HRP/AEC (ABC) IHC Detection Kit ab93705 is a complete kit to detect a primary antibody raised in either mouse or rabbit, using HRP-labeled-streptavidin and a biotinylated anti-rabbit/anti-mouse secondary antibody.


    It is supplied with a full protocol and with peroxidase and protein blocking reagents, biotinylated secondary antibody, streptavidin-HRP, and AEC substrate.


    In the method:
    - a sample is incubated with an unconjugated primary antibody specific to the target antigen
    - a biotinylated secondary antibody then binds to the primary antibody
    - HRP-labeled streptavidin then binds to the secondary antibody
    - the HRP produces a colored substance at the site of primary antibody binding by reacting with AEC.

  • Notes

    For alternative AEC streptavidin-biotin IHC detection kits see:
    - anti-Rabbit HRP-AEC IHC kit ab64260 
    - Anti-Mouse HRP-AEC IHC Kit ab64258

    For high sensitivity AEC HRP micro-polymer IHC detection kits see:
    - Anti-Mouse / anti-Rabbit HRP-AEC Micro-polymer IHC Kit ab236467
    - Anti-Rabbit HRP-AEC Micro-polymer IHC Kit ab236468

    Alternatively, see HRP-DAB IHC detection kits.

    Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

  • Tested applications

    Suitable for: IHC-Pmore details

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 15 ml
    AEC Single Solution 1 x 15ml
    Biotinylated Goat Anti-Polyvalent 1 x 15ml
    Hydrogen Peroxide Block 1 x 15ml
    Protein block 1 x 15ml
    Streptavidin Peroxidase 1 x 15ml
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • IHC Kits
    • Kits/ Lysates/ Other
    • Kits
    • IHC tools
    • ABC
  • Relevance

    An HRP/AEC Detection Kit is a labeled immunoenzymatic antigen detection system. Horseradish peroxidase (HRP) readily combines with hydrogen peroxide (H2O2) and the resultant complex can oxidize a wide variety of chromogenic substrates. AEC is 3-amino-9-ethyl-carbazole, a chromogenic substrate.
  • Alternative names

    • carbazole
    • Horseradish Peroxidase

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab93705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent dilution.
Notes
IHC-P
Use at an assay dependent dilution.

Images

  • CD34 detection using HRP/AEC Detection Kit (ab93705)
    CD34 detection using HRP/AEC Detection Kit (ab93705)This image is courtesy of an Abreview submitted by Stanislava Reinstein Merjava

    Human cornea (cryosections fixed in cold acetone) stained with anti-CD34 antibody using ab93705 Mouse and Rabbit Specific HRP/AEC detection IHC kit.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/AEC detection IHC kit  (ab93705)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/AEC detection IHC kit (ab93705)
    Human tonsil stained with anti-CD3 antibody using ab93705 Polyvalent HRP/AEC Detection Kit (Ready-To-Use).

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (11)

Publishing research using ab93705? Please let us know so that we can cite the reference in this datasheet.

ab93705 has been referenced in 11 publications.

  • Yenmis G  et al. Metformin promotes apoptosis in primary breast cancer cells by downregulation of cyclin D1 and upregulation of P53 through an AMPK-alpha independent mechanism Turk J Med Sci 51:826-834 (2021). PubMed: 33350292
  • Soydas T  et al. Metformin reverses the effects of high glucose on human dermal fibroblasts of aged skin via downregulating RELA/p65 expression. J Physiol Biochem N/A:N/A (2021). PubMed: 34129225
  • Liang YC  et al. ADRM1 as a therapeutic target in hepatocellular carcinoma. Kaohsiung J Med Sci 37:47-54 (2021). PubMed: 32916039
  • Nukpook T  et al. Prevalence and association of Epstein-Barr virus infection with sinonasal inverted papilloma and sinonasal squamous cell carcinoma in the northeastern Thai population. Infect Agent Cancer 15:43 (2020). PubMed: 32595759
  • Coskun G  et al. Effects of Osteocalcin on Synthesis of Testosterone and INSL3 during Adult Leydig Cell Differentiation. Int J Endocrinol 2019:1041760 (2019). PubMed: 31558901
View all Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

CD34 detection using HRP/AEC Detection Kit (ab93705).

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Human cornea stained with anti-CD34 antibody using ab93705 Polyvalent HRP/AEC Detection Kit (Ready-To-Use).
Fig.: Immunohistochemistry (Cryosections fixed in cold acetone) - Mouse and Rabbit Specific HRP/AEC detection IHC kit (ab93705).

Stanislava Reinstein Merjava
Laboratory of the Biology and Pathology of the Eye
Institute of Inherited Metabolic Disorders
Charles University, First Faculty of Medicine and General University Hospital
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Stanislava Reinstein Merjava

Verified customer

Submitted Oct 06 2015

Question

One of our customers wants to use ab93705 kit for IHC on Mouse samples.
The secondary antibody included with the kit is anti Mouse/Rabbit, I'm concerned it might react with the mouse tissue and result in unspecific staining.
Is the suitable for Mouse derived samples and would the included blocking reagent prevent unspecific staining? Is there a more suitable kit for this purpose?

Read More

Abcam community

Verified customer

Asked on Apr 11 2013

Answer



Our ab93705 (Mouse and Rabbit Specific HRP/AEC (ABC) Detection IHC Kit) is not suitable for use on mouse samples. The secondary antibody is a cocktail of biotinylated anti-mouse and anti-rabbit antibodies and the anti-mouse antibodies will react with endogenous Ig in the mouse samples. The blocking reagent will not prevent that staining.

If the customer is using a rabbit primary antibody I would recommend that they use our ab64260 (Rabbit specific HRP/AEC (ABC) Detection IHC Kit) instead:

https://www.abcam.com/Rabbit-specific-HRPAEC-ABC-Detection-IHC-Kit-ab64260.html (or use the following: https://www.abcam.com/Rabbit-specific-HRPAEC-ABC-Detection-IHC-Kit-ab64260.html).

Read More

Abcam Scientific Support

Answered on Apr 11 2013

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