Mouse and Rabbit Specific HRP/AEC (ABC) Detection IHC Kit (ab93705)

Reviews (1)Q&A (1)References (10)
CD34 detection using HRP/AEC Detection Kit (ab93705)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/AEC detection IHC kit (ab93705)

Overview

  • Product name

    Mouse and Rabbit Specific HRP/AEC (ABC) Detection IHC Kit
    See all HRP/AEC Detection Kit kits

  • Product overview

    Mouse and Rabbit specific HRP/AEC (ABC) IHC Detection Kit ab93705 is a complete kit to detect a primary antibody raised in either mouse or rabbit, using HRP-labeled-streptavidin and a biotinylated anti-rabbit/anti-mouse secondary antibody.


    It is supplied with a full protocol and with peroxidase and protein blocking reagents, biotinylated secondary antibody, streptavidin-HRP, and AEC substrate.


    In the method:
    - a sample is incubated with an unconjugated primary antibody specific to the target antigen
    - a biotinylated secondary antibody then binds to the primary antibody
    - HRP-labeled streptavidin then binds to the secondary antibody
    - the HRP produces a colored substance at the site of primary antibody binding by reacting with AEC.

  • Notes

    For alternative AEC streptavidin-biotin IHC detection kits see:
    - anti-Rabbit HRP-AEC IHC kit ab64260 
    - Anti-Mouse HRP-AEC IHC Kit ab64258

    For high sensitivity AEC HRP micro-polymer IHC detection kits see:
    - Anti-Mouse / anti-Rabbit HRP-AEC Micro-polymer IHC Kit ab236467
    - Anti-Rabbit HRP-AEC Micro-polymer IHC Kit ab236468

    Alternatively, see HRP-DAB IHC detection kits.

    Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

  • Tested applications

    Suitable for: IHC-Pmore details

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 15 ml
    AEC Single Solution 1 x 15ml
    Biotinylated Goat Anti-Polyvalent 1 x 15ml
    Hydrogen Peroxide Block 1 x 15ml
    Protein block 1 x 15ml
    Streptavidin Peroxidase 1 x 15ml

  • Research areas

  • Relevance

    An HRP/AEC Detection Kit is a labeled immunoenzymatic antigen detection system. Horseradish peroxidase (HRP) readily combines with hydrogen peroxide (H2O2) and the resultant complex can oxidize a wide variety of chromogenic substrates. AEC is 3-amino-9-ethyl-carbazole, a chromogenic substrate.

  • Alternative names

    • carbazole
    • Horseradish Peroxidase

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab93705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent dilution.
Notes
IHC-P
Use at an assay dependent dilution.

Images

  • CD34 detection using HRP/AEC Detection Kit (ab93705)
    CD34 detection using HRP/AEC Detection Kit (ab93705)This image is courtesy of an Abreview submitted by Stanislava Reinstein Merjava

    Human cornea (cryosections fixed in cold acetone) stained with anti-CD34 antibody using ab93705 Mouse and Rabbit Specific HRP/AEC detection IHC kit.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/AEC detection IHC kit (ab93705)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/AEC detection IHC kit (ab93705)
    Human tonsil stained with anti-CD3 antibody using ab93705 Polyvalent HRP/AEC Detection Kit (Ready-To-Use).

References (10)

Publishing research using ab93705? Please let us know so that we can cite the reference in this datasheet.

ab93705 has been referenced in 10 publications.

  • Yenmis G  et al. Metformin promotes apoptosis in primary breast cancer cells by downregulation of cyclin D1 and upregulation of P53 through an AMPK-alpha independent mechanism Turk J Med Sci 51:826-834 (2021). PubMed: 33350292
  • Soydas T  et al. Metformin reverses the effects of high glucose on human dermal fibroblasts of aged skin via downregulating RELA/p65 expression. J Physiol Biochem N/A:N/A (2021). PubMed: 34129225
  • Nukpook T  et al. Prevalence and association of Epstein-Barr virus infection with sinonasal inverted papilloma and sinonasal squamous cell carcinoma in the northeastern Thai population. Infect Agent Cancer 15:43 (2020). PubMed: 32595759
  • Coskun G  et al. Effects of Osteocalcin on Synthesis of Testosterone and INSL3 during Adult Leydig Cell Differentiation. Int J Endocrinol 2019:1041760 (2019). PubMed: 31558901
  • Wu N  et al. RCC2 over-expression in tumor cells alters apoptosis and drug sensitivity by regulating Rac1 activation. BMC Cancer 18:67 (2018). PubMed: 29321004
  • Ho SS  et al. Hypoxic Preconditioning of Mesenchymal Stem Cells with Subsequent Spheroid Formation Accelerates Repair of Segmental Bone Defects. Stem Cells 36:1393-1403 (2018). PubMed: 29968952
  • Ruan L  et al. SIRT1 contributes to neuroendocrine differentiation of prostate cancer. Oncotarget 9:2002-2016 (2018). PubMed: 29416748
  • Ho SS  et al. Cell Migration and Bone Formation from Mesenchymal Stem Cell Spheroids in Alginate Hydrogels Are Regulated by Adhesive Ligand Density. Biomacromolecules 18:4331-4340 (2017). PubMed: 29131587
  • Friedman O  et al. Immunological and inflammatory mapping of vascularized composite allograft rejection processes in a rat model. PLoS One 12:e0181507 (2017). PubMed: 28746417
  • Gao Y  et al. Adrenomedullin blockade suppresses sunitinib-resistant renal cell carcinoma growth by targeting the ERK/MAPK pathway. Oncotarget 7:63374-63387 (2016). PubMed: 27556517

Customer reviews and Q&As

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CD34 detection using HRP/AEC Detection Kit (ab93705).

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Abreviews
abreview image
Human cornea stained with anti-CD34 antibody using ab93705 Polyvalent HRP/AEC Detection Kit (Ready-To-Use).
Fig.: Immunohistochemistry (Cryosections fixed in cold acetone) - Mouse and Rabbit Specific HRP/AEC detection IHC kit (ab93705).

Stanislava Reinstein Merjava
Laboratory of the Biology and Pathology of the Eye
Institute of Inherited Metabolic Disorders
Charles University, First Faculty of Medicine and General University Hospital

Stanislava Reinstein Merjava

Verified customer

Submitted Oct 06 2015

Question

One of our customers wants to use ab93705 kit for IHC on Mouse samples.
The secondary antibody included with the kit is anti Mouse/Rabbit, I'm concerned it might react with the mouse tissue and result in unspecific staining.
Is the suitable for Mouse derived samples and would the included blocking reagent prevent unspecific staining? Is there a more suitable kit for this purpose?

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Answer



Our ab93705 (Mouse and Rabbit Specific HRP/AEC (ABC) Detection IHC Kit) is not suitable for use on mouse samples. The secondary antibody is a cocktail of biotinylated anti-mouse and anti-rabbit antibodies and the anti-mouse antibodies will react with endogenous Ig in the mouse samples. The blocking reagent will not prevent that staining.

If the customer is using a rabbit primary antibody I would recommend that they use our ab64260 (Rabbit specific HRP/AEC (ABC) Detection IHC Kit) instead:

https://www.abcam.com/Rabbit-specific-HRPAEC-ABC-Detection-IHC-Kit-ab64260.html (or use the following: https://www.abcam.com/Rabbit-specific-HRPAEC-ABC-Detection-IHC-Kit-ab64260.html).

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