Mouse and Rabbit Specific HRP/AEC (ABC) Detection IHC Kit (ab93705)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Mouse and Rabbit Specific HRP/AEC (ABC) Detection IHC Kit
See all HRP/AEC Detection Kit kits -
Product overview
Mouse and Rabbit specific HRP/AEC (ABC) IHC Detection Kit ab93705 is a complete kit to detect a primary antibody raised in either mouse or rabbit, using HRP-labeled-streptavidin and a biotinylated anti-rabbit/anti-mouse secondary antibody.
It is supplied with a full protocol and with peroxidase and protein blocking reagents, biotinylated secondary antibody, streptavidin-HRP, and AEC substrate.
In the method:
- a sample is incubated with an unconjugated primary antibody specific to the target antigen
- a biotinylated secondary antibody then binds to the primary antibody
- HRP-labeled streptavidin then binds to the secondary antibody
- the HRP produces a colored substance at the site of primary antibody binding by reacting with AEC. -
Notes
For alternative AEC streptavidin-biotin IHC detection kits see:
- anti-Rabbit HRP-AEC IHC kit ab64260
- Anti-Mouse HRP-AEC IHC Kit ab64258For high sensitivity AEC HRP micro-polymer IHC detection kits see:
- Anti-Mouse / anti-Rabbit HRP-AEC Micro-polymer IHC Kit ab236467
- Anti-Rabbit HRP-AEC Micro-polymer IHC Kit ab236468
Alternatively, see HRP-DAB IHC detection kits.Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
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Tested applications
Suitable for: IHC-Pmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 15 ml AEC Single Solution 1 x 15ml Biotinylated Goat Anti-Polyvalent 1 x 15ml Hydrogen Peroxide Block 1 x 15ml Protein block 1 x 15ml Streptavidin Peroxidase 1 x 15ml -
Research areas
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Relevance
An HRP/AEC Detection Kit is a labeled immunoenzymatic antigen detection system. Horseradish peroxidase (HRP) readily combines with hydrogen peroxide (H2O2) and the resultant complex can oxidize a wide variety of chromogenic substrates. AEC is 3-amino-9-ethyl-carbazole, a chromogenic substrate. -
Alternative names
- carbazole
- Horseradish Peroxidase
Applications
Our Abpromise guarantee covers the use of ab93705 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent dilution. |
Images
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CD34 detection using HRP/AEC Detection Kit (ab93705)This image is courtesy of an Abreview submitted by Stanislava Reinstein MerjavaHuman cornea (cryosections fixed in cold acetone) stained with anti-CD34 antibody using ab93705 Mouse and Rabbit Specific HRP/AEC detection IHC kit.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/AEC detection IHC kit (ab93705)Human tonsil stained with anti-CD3 antibody using ab93705 Polyvalent HRP/AEC Detection Kit (Ready-To-Use).
Protocols
References
This product has been referenced in:
- Wu N et al. RCC2 over-expression in tumor cells alters apoptosis and drug sensitivity by regulating Rac1 activation. BMC Cancer 18:67 (2018). Read more (PubMed: 29321004) »
- Ho SS et al. Hypoxic Preconditioning of Mesenchymal Stem Cells with Subsequent Spheroid Formation Accelerates Repair of Segmental Bone Defects. Stem Cells 36:1393-1403 (2018). Read more (PubMed: 29968952) »
