Mouse and Rabbit Specific HRP/AEC (ABC) Detection IHC Kit (ab93705)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameMouse and Rabbit Specific HRP/AEC (ABC) Detection IHC Kit
See all HRP/AEC Detection Kit kits -
Product overview
The reagents in this kit constitute a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.
Reacts with Mouse and Rabbit IgG (H+L)
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Tested applicationsSuitable for: IHC-Pmore details
Properties
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Storage instructionsStore at +4°C. Please refer to protocols.
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Components 15 ml AEC Single Solution 1 x 15ml Biotinylated Goat Anti-Polyvalent 1 x 15ml Hydrogen Peroxide Block 1 x 15ml Protein block 1 x 15ml Streptavidin Peroxidase 1 x 15ml -
Research areas
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RelevanceAn HRP/AEC Detection Kit is a labeled immunoenzymatic antigen detection system. Horseradish peroxidase (HRP) readily combines with hydrogen peroxide (H2O2) and the resultant complex can oxidize a wide variety of chromogenic substrates. AEC is 3-amino-9-ethyl-carbazole, a chromogenic substrate.
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Alternative names
- carbazole
- Horseradish Peroxidase
Applications
Our Abpromise guarantee covers the use of ab93705 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent dilution. |
Images
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CD34 detection using HRP/AEC Detection Kit (ab93705)This image is courtesy of an Abreview submitted by Stanislava Reinstein MerjavaHuman cornea (cryosections fixed in cold acetone) stained with anti-CD34 antibody using ab93705 Mouse and Rabbit Specific HRP/AEC detection IHC kit.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/AEC detection IHC kit (ab93705)Human tonsil stained with anti-CD3 antibody using ab93705 Polyvalent HRP/AEC Detection Kit (Ready-To-Use).
Protocols
References
This product has been referenced in:
- Wu N et al. RCC2 over-expression in tumor cells alters apoptosis and drug sensitivity by regulating Rac1 activation. BMC Cancer 18:67 (2018). Read more (PubMed: 29321004) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"