Mouse and Rabbit Specific HRP/AEC IHC Detection Kit - Micro-polymer (ab236467)

Overview

  • Product name

    Mouse and Rabbit Specific HRP/AEC IHC Detection Kit - Micro-polymer
  • Detection method

    Colorimetric
  • Assay type

    Micro-Polymer
  • Product overview

    This IHC detection kit is used to detect mouse and rabbit primary antibodies in immunohistochemistry (IHC). It uses an HRP micro-polymer secondary antibody for highly sensitive detection.


    HRP micro-polymer methods are more sensitive than the traditional streptavidin-biotin method, and they do not require a blocking step for endogenous biotin.


    This kit is an exact copy of EXPOSE Mouse and Rabbit Specific HRP/AEC Detection IHC Kit ab93686, except that it uses the HRP micro-polymer antibody used during the validation of Abcam's antibodies in IHC. This HRP micro-polymer antibody was identified by our R&D team as giving the most sensitive and specific staining.


    The kit includes ready-to-use hydrogen peroxide blocking reagent, protein blocking reagent, HRP micro-polymer conjugated goat anti-rabbit secondary antibody, and AEC chromogen. It includes an unconjugated rabbit anti-mouse antibody, which is used together with the HRP polymer anti-rabbit antibody to detect mouse primary antibodies.

  • Notes

    Other IHC detection products

    For alternative kits, see Anti-Rabbit HRP-AEC Micro-polymer IHC Kit ab236468, and HRP-DAB micro-polymer IHC kits.

    For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055.

    Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

  • Tested applications

    Suitable for: IHC-Pmore details

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components Identifier 15 ml 125 ml
    AEC Single Solution 1 x 15ml
    Goat anti-rabbit HRP Conjugate 1 x 15ml
    Hydrogen Peroxide Block 1 x 15ml
    Mouse Specifying Reagent (Complement) 1 x 15ml
    Protein block 1 x 15ml
  • Research areas

  • Relevance

    Biotin-Free Polyvalent AEC detection is a biotin-free immunoenzymatic antigen detection system. AEC is 3-amino-9-ethyl-carbazole, a chromogenic substrate.

Associated products

Applications

Our Abpromise guarantee covers the use of ab236467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Images

  • Immunohistochemical staining of p53 in human colon adenocarcinoma with anti-p53 antibody at a dilution of 1/400 and detected using ab93686 (this kit is identical to ab93686, except that it uses the same HRP micro-polymer antibody used by Abcam's R&D team for antibody validation in IHC.

  • Immunohistochemical staining of smooth muscle actin in human small intestine with ab7817 at a dilution of 1/200 and detected using ab93686 (this kit is identical to ab93686, except that it uses the HRP micro-polymer secondary antibody used by Abcam's R&D team for antibody validation in IHC).

Protocols

References

ab236467 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab236467.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up