Mouse and Rabbit Specific HRP/DAB (ABC) Detection IHC kit (ab64264)

Abreviews (2)Q&A (10)Specific References (27)

Overview

  • Product name
    Mouse and Rabbit Specific HRP/DAB (ABC) Detection IHC kit
    See all HRP/DAB detection kit kits
  • Product overview

    The reagents in this kit constitute a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.

  • Notes

    DAB is a suspected carcinogen. Handle with care.

    Contains hydrogen peroxide.

  • Tested applications
    Suitable for: IHC-Pmore details

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 15 ml 15 ml
    50x DAB Chromogen 1 x 0.5ml 1 x 0.5ml
    Biotinylated Goat Anti-Polyvalent 1 x 15ml 1 x 15ml
    DAB substrate 1 x 15ml 1 x 15ml
    Hydrogen Peroxide Block 1 x 15ml 1 x 15ml
    Protein block 1 x 15ml 1 x 15ml
    Streptavidin Peroxidase 1 x 15ml 1 x 15ml
  • Research areas
  • Relevance
    HRP/DAB detection kit is a immunoenzymatic antigen detection system for immunohistochemistry techniques.

Applications

Our Abpromise guarantee covers the use of ab64264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

Images

  • Immunohistochemistry - ab64264
    Immunohistochemistry - ab64264Yu S.H., PLoS One 9 (12), Fig 7c. doi: 10.1371/journal.pone.0114649

    Immunohistochemical analysis staining CD163 in mouse soleus muscle section. Samples were incubated in rabbit CD163 antibody at 1:450 dilution and visualized using mouse and rabbit specific HRP/DAB detection IHC Kit (ab64264).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti Mouse & Rabbit HRP/DAB Detection Kit (ab64264)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti Mouse & Rabbit HRP/DAB Detection Kit (ab64264)

    Staining of human estrogen receptor in breast carcinoma tissue using ab16660 - Rabbit monoclonal [SP1] to Estrogen Receptor, and ab64246 - Mouse & Rabbit HRP/DAB Detection Kit.

References

This product has been referenced in:
  • Tong WY  et al. Delivery of siRNA in vitro and in vivo using PEI-capped porous silicon nanoparticles to silence MRP1 and inhibit proliferation in glioblastoma. J Nanobiotechnology 16:38 (2018). Read more (PubMed: 29653579) »
  • Nordquist E  et al. Postnatal and Adult Aortic Heart Valves Have Distinctive Transcriptional Profiles Associated With Valve Tissue Growth and Maintenance Respectively. Front Cardiovasc Med 5:30 (2018). IHC-P . Read more (PubMed: 29740591) »

See all 27 Publications for this product

Publishing research using ab64264? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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12 reviews or Q&A

10
Votes: Highest First




Thank you for all the advice and the help you gave me

Please I was reading the protocol of the IHC kit I bought and I would like to have more information about the 3rd step in the staining protocol when you speak about perfoming...

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The pretreatment note in the protocol of the IHC kit refers to the antigen retrieval step that is required for most antibody to achieve a successful IHC-P staining.
For both of these antibodies (ab14440 and ab52894) I can recommend to use Sod...

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IHC-P Mouse pancreas

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
Diseased mouse pancreas tissue labeled with REST / NRSF (ab202962) at 1/100 dilution - 1 hr RT, followed by conjugation to the secondary antibody and DAB staining
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Verified customer

Submitted Aug 04 2016

Anti-CCR2 antibody

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
Detection of anti-CCR2 (monoclonal anti-rabbit) in human paraffin sections using HRP/DAB detection kit.
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Abcam user community

Verified customer

Submitted Feb 10 2016

Thank you for your support. Customer will chose one from ab113316
and ab101854. But customer will not able to buy ab80436 and ab64264
kit together. So he want to know which one would be better among this two
kit for his work. Please sugges...

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This is confusing. ab113316 is a kit not antibody, customer would not be requiring a DAB kit for this.

For ab101854, ab80436 will be a good product.

Thanks!

One of our customer has selected two primary antibody(Ab10148, Ab75714)
for IHC stainning on Alzheimer's disease in rat model. And his interest is to stain
brain area of Prefrontal cortex, Hippocampus, Hypothalamus, Cerebellum, Pons
& M...

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Thank you for contacting us.

The kits ab80436 and ab64264, both will be OK to use with ab10148 only because it is rabbit antibody, you can select any one of them. Please note that these kits cannot be used with ab75714 because it is a chicken ...

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Please confirm me whether I can use Mouse and Rabbit Specific HRP/DAB (ABC) detection IHC kit (ab64264) and AEC Substrate System (Ready to Use) (ab64252)
for rat sections. Most of the research is carried out in rat and mice,it will be helpful for t...

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Yes, Mouse and Rabbit Specific HRP/DAB (ABC) detection IHC kit (ab64264) and AEC Substrate System (Ready to Use) (ab64252) can be used with rat sections.

Dears,

sorry for many questions :)

Is Mouse and Rabbit Specific HRP/DAB (ABC) detection IHC kit (ab64264) need anything else to complete full IHC Experiment with our antobodies. I mean like if the customer needs antibody diluent o...

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Thank you for your enquiry.

The list of components of this kit can be found on the online datasheet:
https://www.abcam.com/index.html?datasheet=64264 (or use the following: https://www.abcam.com/index.html?datasheet=64264).

5...

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Inquiry: i am using ab292 for staining bone marrow section formalin fixed parrafin embedded . i read the protocol used for the human adrenal gland found attached to the antibody sheet .i have some questions please: 1-if i am going to use the ab64264 as...

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Thank you for contacting us.

The protocol mentioned described on the datasheet refers to an Abreview from a previous user of this antibody. Different protocols may also work.

If you are using ab64264 kit, there is no need to use an ...

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Dear Sir or Madame,

Could you please inform me, whether is possible to order a DAB substrate alone in the Mouse and Rabbit specific HRP/DAB detection IHC kit (ab64264)?

Thank you very much

Thank you for your enquiry which has been forwarded to the Scientific support team.

I am sorry, it is regrettably not possible toprovide this particular component separately.

If you have any further questions, please do not hesita...

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Dear All,



Please let us know about the following that many slides should have been available for the concentrated antibodies after dilution and how many slides can be prepeared with the prediluted slides. Also let us know the abo...

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Thank you for your inquiry.

Since the working dilutions of antibodies are not a fixed value but have to optimised by the endusers themselves according to experimental condition, I cannot give you absolute numbers. Even when the pre-diluted an...

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1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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