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The reagents in this kit constitute a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.
DAB is a suspected carcinogen. Handle with care.
Contains hydrogen peroxide.
|Components||15 ml||15 ml|
|50x DAB Chromogen||1 x 0.5ml||1 x 0.5ml|
|Biotinylated Goat Anti-Polyvalent||1 x 15ml||1 x 15ml|
|DAB substrate||1 x 15ml||1 x 15ml|
|Hydrogen Peroxide Block||1 x 15ml||1 x 15ml|
|Protein block||1 x 15ml||1 x 15ml|
|Streptavidin Peroxidase||1 x 15ml||1 x 15ml|
Our Abpromise guarantee covers the use of ab64264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution.|
Immunohistochemical analysis staining CD163 in mouse soleus muscle section. Samples were incubated in rabbit CD163 antibody at 1:450 dilution and visualized using mouse and rabbit specific HRP/DAB detection IHC Kit (ab64264).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"