Mouse and Rabbit Specific HRP/DAB (ABC) Detection IHC kit (ab64264)
- Datasheet
- References (46)
- Protocols
Overview
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Product name
Mouse and Rabbit Specific HRP/DAB (ABC) Detection IHC kit
See all HRP/DAB detection kit kits -
Product overview
Mouse and Rabbit specific HRP/DAB (ABC) Detection IHC Kit ab64264 is a complete kit to detect a primary antibody raised in either mouse or rabbit, using HRP-labeled-streptavidin and a biotinylated anti-rabbit/anti-mouse secondary antibody.
It is supplied with a full protocol and with peroxidase and protein blocking reagents, biotinylated secondary antibody, streptavidin-HRP, and DAB substrate.
In the method:
- a sample is incubated with an unconjugated primary antibody specific to the target antigen
- a biotinylated secondary antibody then binds to the primary antibody
- HRP-labeled streptavidin then binds to the secondary antibody
- the HRP produces a brown colored substance at the site of primary antibody binding by reacting with DAB. -
Notes
For alternative streptavidin-biotin IHC detection kits see:
- Anti-Rabbit HRP-DAB IHC kit ab64261
- Anti-Mouse HRP-DAB IHC Kit ab64259For high sensitivity HRP micro-polymer IHC detection kits see:
- Anti-Mouse / anti-Rabbit HRP/DAB Micro-polymer IHC Kit ab236466
- Anti-Rabbit HRP-DAB Micro-polymer IHC Kit ab236469Alternatively, see HRP-AEC IHC detection kits.
For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
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Tested applications
Suitable for: IHC-Pmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 15 ml 50x DAB Chromogen 1 x 0.5ml Biotinylated Goat Anti-Polyvalent 1 x 15ml DAB substrate 1 x 15ml Hydrogen Peroxide Block 1 x 15ml Protein block 1 x 15ml Streptavidin Peroxidase 1 x 15ml -
Research areas
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Relevance
HRP/DAB detection kit is a immunoenzymatic antigen detection system for immunohistochemistry techniques.
Applications
Our Abpromise guarantee covers the use of ab64264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent dilution. |
Images
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Immunohistochemistry - ab64264Yu S.H., PLoS One 9 (12), Fig 7c. doi: 10.1371/journal.pone.0114649 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunohistochemical analysis staining CD163 in mouse soleus muscle section. Samples were incubated in rabbit CD163 antibody at 1:450 dilution and visualized using mouse and rabbit specific HRP/DAB detection IHC Kit (ab64264).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti Mouse & Rabbit HRP/DAB Detection Kit (ab64264)
References
This product has been referenced in:
- Han R et al. Upregulated long noncoding RNA LOC105375913 induces tubulointerstitial fibrosis in focal segmental glomerulosclerosis. Sci Rep 9:716 (2019). Read more (PubMed: 30679767) »
- Hamzeh M et al. Cerium Oxide Nanoparticles Protect Cyclophosphamide-induced Testicular Toxicity in Mice. Int J Prev Med 10:5 (2019). Read more (PubMed: 30774839) »
