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  1. Link

    mouse-and-rabbit-specific-hrpdab-abc-detection-ihc-kit-ab64264.pdf

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Mouse and Rabbit Specific HRP/DAB (ABC) Detection IHC kit (ab64264)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (3)Q&A (10)References (115)

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Immunohistochemistry - ab64264
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti Mouse & Rabbit HRP/DAB Detection Kit  (ab64264)

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Overview

  • Product name

    Mouse and Rabbit Specific HRP/DAB (ABC) Detection IHC kit
    See all HRP/DAB detection kit kits
  • Product overview

    Mouse and Rabbit specific HRP/DAB (ABC) Detection IHC Kit ab64264 is a complete kit to detect a primary antibody raised in either mouse or rabbit, using HRP-labeled-streptavidin and a biotinylated anti-rabbit/anti-mouse secondary antibody.


    It is supplied with a full protocol and with peroxidase and protein blocking reagents, biotinylated secondary antibody, streptavidin-HRP, and DAB substrate.


    In the method:
    - a sample is incubated with an unconjugated primary antibody specific to the target antigen
    - a biotinylated secondary antibody then binds to the primary antibody
    - HRP-labeled streptavidin then binds to the secondary antibody
    - the HRP produces a brown colored substance at the site of primary antibody binding by reacting with DAB.

  • Notes

    For alternative streptavidin-biotin IHC detection kits see:
    - Anti-Rabbit HRP-DAB IHC kit ab64261
    - Anti-Mouse HRP-DAB IHC Kit ab64259

    For high sensitivity HRP micro-polymer IHC detection kits see:
    - Anti-Mouse / anti-Rabbit HRP/DAB Micro-polymer IHC Kit ab236466
    - Anti-Rabbit HRP-DAB Micro-polymer IHC Kit ab236469

    Alternatively, see HRP-AEC IHC detection kits.

    For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055.

    Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

  • Tested applications

    Suitable for: IHC-Pmore details

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 15 ml
    50x DAB Chromogen 1 x 0.5ml
    Biotinylated Goat Anti-Polyvalent 1 x 15ml
    DAB substrate 1 x 15ml
    Hydrogen Peroxide Block 1 x 15ml
    Protein block 1 x 15ml
    Streptavidin Peroxidase 1 x 15ml
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • IHC Kits
    • Kits/ Lysates/ Other
    • Kits
    • IHC tools
    • ABC
  • Relevance

    HRP/DAB detection kit is a immunoenzymatic antigen detection system for immunohistochemistry techniques.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab64264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent dilution.
Notes
IHC-P
Use at an assay dependent dilution.

Images

  • Immunohistochemistry - ab64264
    Immunohistochemistry - ab64264Yu S.H., PLoS One 9 (12), Fig 7c. doi: 10.1371/journal.pone.0114649 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunohistochemical analysis staining CD163 in mouse soleus muscle section. Samples were incubated in rabbit CD163 antibody at 1:450 dilution and visualized using mouse and rabbit specific HRP/DAB detection IHC Kit (ab64264).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti Mouse & Rabbit HRP/DAB Detection Kit  (ab64264)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti Mouse & Rabbit HRP/DAB Detection Kit (ab64264)

    Staining of human estrogen receptor in breast carcinoma tissue using ab16660 - Rabbit monoclonal [SP1] to Estrogen Receptor, and ab64246 - Mouse & Rabbit HRP/DAB Detection Kit.

Protocols

  • Polyvalent HRP/DAB detection kit
  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (115)

Publishing research using ab64264? Please let us know so that we can cite the reference in this datasheet.

ab64264 has been referenced in 115 publications.

  • Gor R  et al. RNA binding protein PUM1 promotes colon cancer cell proliferation and migration. Int J Biol Macromol 174:549-561 (2021). PubMed: 33508364
  • Xin S  et al. Isolation, genotyping and virulence determination of a Toxoplasma gondii strain from non-human primate from China. Transbound Emerg Dis N/A:N/A (2021). PubMed: 33619895
  • Fuselier C  et al. Anti-Tumoral and Anti-Angiogenic Effects of Low-Diluted Phenacetinum on Melanoma. Front Oncol 11:597503 (2021). PubMed: 33747916
  • Fan Y  et al. Transplantation of human amniotic epithelial cells promotes morphological and functional regeneration in a rat uterine scar model. Stem Cell Res Ther 12:207 (2021). PubMed: 33762002
  • Liu X  et al. CREB1 acts via the miR-922/ARID2 axis to enhance malignant behavior of liver cancer cells. Oncol Rep 45:N/A (2021). PubMed: 33786634
View all Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

ab64264 - Mouse and Rabbit Specific HRP/DAB (ABC) Detection IHC kit

Good Good 4/5 (Ease of Use)
Abreviews
Abreviews
the kit is easy to use and signal is very clear and bright.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 30 2019

IHC-P Mouse pancreas

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Diseased mouse pancreas tissue labeled with REST / NRSF (ab202962) at 1/100 dilution - 1 hr RT, followed by conjugation to the secondary antibody and DAB staining
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 04 2016

Anti-CCR2 antibody

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Detection of anti-CCR2 (monoclonal anti-rabbit) in human paraffin sections using HRP/DAB detection kit.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 10 2016

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