Mouse and Rabbit Specific HRP/DAB (ABC) Detection IHC kit (ab64264)
Overview
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Product name
Mouse and Rabbit Specific HRP/DAB (ABC) Detection IHC kit
See all HRP/DAB detection kit kits -
Product overview
Mouse and Rabbit specific HRP/DAB (ABC) Detection IHC Kit ab64264 is a complete kit to detect a primary antibody raised in either mouse or rabbit, using HRP-labeled-streptavidin and a biotinylated anti-rabbit/anti-mouse secondary antibody.
It is supplied with a full protocol and with peroxidase and protein blocking reagents, biotinylated secondary antibody, streptavidin-HRP, and DAB substrate.
In the method:
- a sample is incubated with an unconjugated primary antibody specific to the target antigen
- a biotinylated secondary antibody then binds to the primary antibody
- HRP-labeled streptavidin then binds to the secondary antibody
- the HRP produces a brown colored substance at the site of primary antibody binding by reacting with DAB. -
Notes
For alternative streptavidin-biotin IHC detection kits see:
- Anti-Rabbit HRP-DAB IHC kit ab64261
- Anti-Mouse HRP-DAB IHC Kit ab64259For high sensitivity HRP micro-polymer IHC detection kits see:
- Anti-Mouse / anti-Rabbit HRP/DAB Micro-polymer IHC Kit ab236466
- Anti-Rabbit HRP-DAB Micro-polymer IHC Kit ab236469Alternatively, see HRP-AEC IHC detection kits.
For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
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Tested applications
Suitable for: IHC-Pmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 15 ml 50x DAB Chromogen 1 x 0.5ml Biotinylated Goat Anti-Polyvalent 1 x 15ml DAB substrate 1 x 15ml Hydrogen Peroxide Block 1 x 15ml Protein block 1 x 15ml Streptavidin Peroxidase 1 x 15ml -
Research areas
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Relevance
HRP/DAB detection kit is a immunoenzymatic antigen detection system for immunohistochemistry techniques.
Associated products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent dilution.
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| Notes |
|---|
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IHC-P
Use at an assay dependent dilution. |
Images
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Immunohistochemistry - ab64264Yu S.H., PLoS One 9 (12), Fig 7c. doi: 10.1371/journal.pone.0114649 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Immunohistochemical analysis staining CD163 in mouse soleus muscle section. Samples were incubated in rabbit CD163 antibody at 1:450 dilution and visualized using mouse and rabbit specific HRP/DAB detection IHC Kit (ab64264).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti Mouse & Rabbit HRP/DAB Detection Kit (ab64264)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (69)
ab64264 has been referenced in 69 publications.
- Meftahi GH et al. Chronic administration of methylphenidate did not affect memory and GDNF levels but increase astrogliosis in adult male rat's hippocampus. J Chem Neuroanat 108:101818 (2020). PubMed: 32485223
- Soltani R et al. Tramadol exposure upregulated apoptosis, inflammation and autophagy in PC12 cells and rat's striatum: An in vitro- in vivo approach. J Chem Neuroanat 109:101820 (2020). PubMed: 32526246
- Podmolíková L et al. Radiation of the urinary bladder attenuates the development of lipopolysaccharide-induced cystitis. Int Immunopharmacol 83:106334 (2020). PubMed: 32179244
- Ma XL et al. Upregulation of RND3 Affects Trophoblast Proliferation, Apoptosis, and Migration at the Maternal-Fetal Interface. Front Cell Dev Biol 8:153 (2020). PubMed: 32232044
- Medeiros B et al. Triple-Negative Primary Breast Tumors Induce Supportive Premetastatic Changes in the Extracellular Matrix and Soluble Components of the Lung Microenvironment. Cancers (Basel) 12:N/A (2020). PubMed: 31936750