Mouse and Rabbit Specific HRP/DAB IHC Detection Kit - Micro-polymer (ab236466)

Overview

  • Product name
    Mouse and Rabbit Specific HRP/DAB IHC Detection Kit - Micro-polymer
    See all HRP/DAB detection kit kits
  • Detection method
    Colorimetric
  • Assay type
    Micro-Polymer
  • Product overview

    This IHC kit is used to detect mouse and rabbit primary antibodies in immunohistochemistry (IHC). It uses an HRP micro-polymer secondary antibody for highly sensitive detection.


    HRP micro-polymer methods are more sensitive than the traditional streptavidin-biotin method, and they do not require a blocking step for endogenous biotin.


    This kit is an exact copy of EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC Kit ab80436, except that it uses the HRP micro-polymer antibody used during the validation of Abcam's antibodies in IHC. This HRP micro-polymer antibody was identified by our R&D team as giving the most sensitive and specific staining. 


    The kit includes ready-to-use hydrogen peroxide blocking reagent, protein blocking reagent, HRP micro-polymer conjugated goat anti-rabbit secondary antibody, and DAB chromogen/substrate. It includes an unconjugated rabbit anti-mouse antibody, which is used together with the HRP polymer anti-rabbit antibody to detect mouse primary antibodies.

  • Notes

    The previous version of this kit, the EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC Kit, was used by researchers to stain over 150,000 slides since 2012 (based on volumes of reagent sold).

     

    Other IHC detection products

    For alternative kits, see anti-Rabbit HRP-DAB Micro-polymer IHC Kit ab236469, and HRP-AEC micro-polymer IHC kits.

    For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055.

    Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

  • Tested applications
    Suitable for: IHC-Pmore details

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 15 ml 60 ml 125 ml 125 ml
    50x DAB Chromogen 1 x 0.5ml 1 x 2ml 1 x 4ml 1 x 4ml
    DAB substrate 1 x 15ml 1 x 60ml 1 x 125ml 1 x 125ml
    Goat anti-rabbit HRP Conjugate 1 x 15ml 1 x 60ml 1 x 125ml 1 x 125ml
    Hydrogen Peroxide Block 1 x 15ml 1 x 60ml 1 x 125ml 1 x 125ml
    Mouse Specifying Reagent (Complement) 1 x 15ml 1 x 60ml 1 x 125ml 1 x 125ml
    Protein block 1 x 15ml 1 x 60ml 1 x 125ml 1 x 125ml
  • Research areas
  • Relevance
    HRP/DAB detection kit is a immunoenzymatic antigen detection system for immunohistochemistry techniques.

Applications

Our Abpromise guarantee covers the use of ab236466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Images

  • ab236466 Mouse and Rabbit Specific HRP-DAB Detection IHC Kit on Human Tonsil staining CD20
  • ab209064 at 1:2000 staining TMEM119 antibody in mouse cerebrum tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TMEM119 with ab209064 at 1/2000 dilution followed by the same HRP micro-polymer goat anti-rabbit secondary antibody used in this kit. Positive staining on glial cells in mouse cerebrum is observed. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0) before commencing with IHC staining protocol. Counter stained with hematoxylin.

  • ab1220 staining human kidney sections by IHC-P using EXPOSE IHC detection kit ab80436 (this kit is identical to ab80436, except that it uses the same HRP polymer secondary antibody used by Abcam's R&D team for antibody validation). Formalin fixed paraffin embedded tissue sections were pre-treated using heat mediated antigen retrieval (using a pressure cooker) with sodium citrate buffer (pH 6) for 30 minutes. The section was incubated with ab1220, 5 µg/ml, for 1 hour at room temperature. DAB was used as the chromogen and the section was counterstained with haematoxylin and mounted with DPX.

Protocols

References

ab236466 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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