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    mouse-and-rabbit-specific-hrpdab-ihc-detection-kit-micro-polymer-ab236466.pdf

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Mouse and Rabbit Specific HRP/DAB IHC Detection Kit - Micro-polymer (ab236466)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (1) Submit a question References (16)

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Effect of doxycycline (DOX) treatment on the expression of MMP-2 and MMP-9 in the skin
  • Effect of doxycycline (DOX) treatment on the expression of MMP-2 and MMP-9
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/DAB IHC Detection Kit - Micro-polymer (ab236466)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

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Overview

  • Product name

    Mouse and Rabbit Specific HRP/DAB IHC Detection Kit - Micro-polymer
    See all HRP/DAB detection kit kits
  • Detection method

    Colorimetric
  • Assay type

    Micro-Polymer
  • Product overview

    This IHC kit is used to detect mouse and rabbit primary antibodies in immunohistochemistry (IHC). It uses an HRP micro-polymer secondary antibody for highly sensitive detection.


    HRP micro-polymer methods are more sensitive than the traditional streptavidin-biotin method, and they do not require a blocking step for endogenous biotin.


    This kit is an exact copy of EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC Kit ab80436, except that it uses the HRP micro-polymer antibody used during the validation of Abcam's antibodies in IHC. This HRP micro-polymer antibody was identified by our R&D team as giving the most sensitive and specific staining. 


    The kit includes ready-to-use hydrogen peroxide blocking reagent, protein blocking reagent, HRP micro-polymer conjugated goat anti-rabbit secondary antibody, and DAB chromogen/substrate. It includes an unconjugated rabbit anti-mouse antibody, which is used together with the HRP polymer anti-rabbit antibody to detect mouse primary antibodies.

  • Notes

    The previous version of this kit, the EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC Kit, was used by researchers to stain over 150,000 slides since 2012 (based on volumes of reagent sold).

     

    Other IHC detection products

    For alternative kits, see anti-Rabbit HRP-DAB Micro-polymer IHC Kit ab236469, and HRP-AEC micro-polymer IHC kits.

    For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055.

    Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

  • Tested applications

    Suitable for: IHC-Pmore details

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 15 ml 60 ml 125 ml
    50x DAB Chromogen 1 x 0.5ml 1 x 2ml 1 x 4ml
    DAB substrate 1 x 15ml 1 x 60ml 1 x 125ml
    Goat anti-rabbit HRP Conjugate 1 x 15ml 1 x 60ml 1 x 125ml
    Hydrogen Peroxide Block 1 x 15ml 1 x 60ml 1 x 125ml
    Mouse Specifying Reagent (Complement) 1 x 15ml 1 x 60ml 1 x 125ml
    Protein block 1 x 15ml 1 x 60ml 1 x 125ml
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • IHC Kits
  • Relevance

    HRP/DAB detection kit is a immunoenzymatic antigen detection system for immunohistochemistry techniques.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab236466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration.

Images

  • Effect of doxycycline (DOX) treatment on the expression of MMP-2 and MMP-9 in the skin
    Effect of doxycycline (DOX) treatment on the expression of MMP-2 and MMP-9 in the skinImage from Arash et al., Sci Rep; 10(1):7154. doi: 10.1038/s41598-020-64071-8.

    Effect of doxycycline (DOX) treatment on the expression of MMP-2 and MMP-9 in the skin. (A) The upper panel shows representative MMP-2 staining of skin from CTRL and MFS mice at 12 months of age with or without DOX, and the lower panel shows bar graphs representing the expression of MMP-2 in the skin of CTRL and MFS mice at 12 weeks of age with or without DOX. (B) The upper panel shows representative MMP-9 staining of skin from CTRL and MFS mice at 12 months of age with or without DOX treatment. The lower panel shows bar graphs representing the expression of MMP-9 in the skin of CTRL and MFS mice at 12 weeks of age treated with or without DOX.

  • Effect of doxycycline (DOX) treatment on the expression of MMP-2 and MMP-9
    Effect of doxycycline (DOX) treatment on the expression of MMP-2 and MMP-9Image from Arash et al., Sci Rep; 10(1):7154. doi: 10.1038/s41598-020-64071-8.

    Effect of doxycycline (DOX) treatment on the expression of MMP-2 and MMP-9 in the aorta.Immunohistochemistry staining images and bar graphs representing the expression of (A) MMP-2 and (B) MMP-9 in the aortic roots of CTRL and MFS mice at 12 weeks of age treated with or without DOX.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/DAB IHC Detection Kit - Micro-polymer (ab236466)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/DAB IHC Detection Kit - Micro-polymer (ab236466)
    ab236466 Mouse and Rabbit Specific HRP-DAB Detection IHC Kit on Human Tonsil staining CD20
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    ab209064 at 1:2000 staining TMEM119 antibody in mouse cerebrum tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TMEM119 with ab209064 at 1/2000 dilution followed by the same HRP micro-polymer goat anti-rabbit secondary antibody used in this kit. Positive staining on glial cells in mouse cerebrum is observed. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0) before commencing with IHC staining protocol. Counter stained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    ab1220 staining human kidney sections by IHC-P using EXPOSE IHC detection kit ab80436 (this kit is identical to ab80436, except that it uses the same HRP polymer secondary antibody used by Abcam's R&D team for antibody validation). Formalin fixed paraffin embedded tissue sections were pre-treated using heat mediated antigen retrieval (using a pressure cooker) with sodium citrate buffer (pH 6) for 30 minutes. The section was incubated with ab1220, 5 µg/ml, for 1 hour at room temperature. DAB was used as the chromogen and the section was counterstained with haematoxylin and mounted with DPX.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (16)

Publishing research using ab236466? Please let us know so that we can cite the reference in this datasheet.

ab236466 has been referenced in 16 publications.

  • Bishnu A  et al. Molecular imaging of the kinetics of hyperactivated ERK1/2-mediated autophagy during acquirement of chemoresistance. Cell Death Dis 12:161 (2021). PubMed: 33558461
  • Pereira JNB  et al. Small intestine remodeling in male Goto-Kakizaki rats. Physiol Rep 9:e14755 (2021). PubMed: 33580916
  • Okkay U  et al. Achillea millefolium alleviates testicular damage in paclitaxel-intoxicated rats via attenuation of testicular oxido-inflammatory stress and apoptotic responses. Andrologia 53:e14028 (2021). PubMed: 33650701
  • Huang Y  et al. A Novel, Personalized Drug-Screening System for Platinum-Resistant Ovarian Cancer Patients: A Preliminary Clinical Report. Cancer Manag Res 13:2849-2867 (2021). PubMed: 33833569
  • Topchu I  et al. Musashi 2 (MSI2) expression as an independent prognostic biomarker in non-small cell lung cancer (NSCLC). J Thorac Dis 13:1370-1379 (2021). PubMed: 33841930
View all Publications for this product

Customer reviews and Q&As

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Test Mouse and Rabbit Specific HRP/DAB IHC Detection Kit - Micro-polymer (ab236466)

Good Good 4/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Kit was used on frozen tissue section instead FFPE section. Frozen tissue section was fixed using paraformaldehyde 4% and permeabilized by 1% Triton X-100. PAP Pen (ab2601) was used as hydrophobic barrier around the section. Then, Mouse and rabbit Specific HRP/DAB IHC Detection kit was used according to the protocol. The section was incubated with anti-NCAM1 antibody (ab237708) 1:1000 overnight. DAB of the kit was used as the chromogen and the section was counterstain with haematoxylin and mounted with Mounting Medium for IHC - Aqueous (ab64230)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. Lucille Cartier

Verified customer

Submitted Jun 28 2021

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