Mouse Angiogenesis Antibody Array - Membrane (24 Targets) (ab139697)

Overview

  • Product name
    Mouse Angiogenesis Antibody Array - Membrane (24 Targets)
    See all Angiogenesis Antibody Array antibody arrays
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type
    Semi-quantitative
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    ab139697 is for simultaneous detection of 24 Mouse Angiogenic factors. Suitable for all sample types.



    Targets: Eotaxin, Fas Ligand, bFGF, G-CSF, GM-CSF, IFN-gamma, IGF-II, IL-1 alpha, IL-1 beta, IL-12 p40/p70, IL-12 p70, IL-13, IL-6, IL-9, Leptin, MCP-1, M-CSF, MIG, PF-4, TIMP-1, TIMP-2, TNF-alpha, Thrombopoietin.


    Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.



    Learn more about membrane antibody arrays

  • Notes

    A table listing all of our mouse membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

  • Tested applications
    Suitable for: Multiplex Protein Detectionmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab139697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection Use at an assay dependent concentration.

Images

  • Typical results obtained with Abcam Membrane Antibody Arrays. Image above presents typical images obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

Protocols

References

This product has been referenced in:
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Q&A

Answer

Stripping the membranes and re-probing is not recommended as stripping buffers contain denaturing reagents which can damage the printed array antibodies, especially the positive control spots. As a result, weakened signals may occur. It may be possible to still get some signals and usable data but we cannot guarantee the kit’s performance or quality if the membranes are stripped or the protocol is modified in any way.

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Answer

Thank you for contacting us. These antibodies are not sold individually, only in the array kit.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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