Overview

  • Product name

    Mouse Angiogenin ELISA Kit
    See all Angiogenin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    serum 8 2.3%
    Inter-assay
    Sample n Mean SD CV%
    serum 3 2.6%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    16.7 pg/ml
  • Range

    62.5 pg/ml - 4000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 106 105% - 107%
    Cell culture media 101 100% - 103%
    Heparin Plasma 106 104% - 108%
    EDTA Plasma 107 105% - 110%
    Citrate Plasma 102 92% - 110%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Rat, Rabbit, Goat, Guinea pig, Hamster, Cow, Dog, Human, Pig
  • Product overview

    Abcam’s Angiogenin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Angiogenin protein in mouse serum, plasma, and cell culture supernatants.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Mouse Angiogenin is a member of the pancreatic ribonuclease A superfamily and is a potent inducer of neovascularization. Furthermore, Angiogenin is essential for cell growth and proliferation. Angiogenin direct binding to the promoter region of ribosomal DNA induces ribosomal RNA transcription and cell proliferation required for ribosomal biogenesis and the action of angiogenic factors. Increased Angiogenin serum levels have been associated with the incidence and severity of several types of tumors. Mouse Angiogenin is 145 amino acids (aa) in length, with a 24 aa signal peptide, and contains three intra-chain disulfide bonds. Human and rat Angiogenin are 74% and 83% identical to mouse Angiogenin, respectively.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab208349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Angiogenin were measured in duplicates, interpolated from the Angiogenin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1.25%, mouse liver supernatant 20% and cell culture media 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiogenin concentration was determined to be 295.7 ng/mL in serum, 16.2 ng/mL in mouse liver supernatant and 10.3 ng/mL in cell culture media. Mouse liver was cultured for 5 days in RPMI base media with 10% fetal bovine serum and supernatants were collected according to section 11.3.

  • The concentrations of Angiogenin were measured in duplicates, interpolated from the Angiogenin standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (citrate) 1%, plasma (EDTA) 1.25%, and plasma (heparin) 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiogenin concentration was determined to be 342.4 ng/mL in plasma (citrate), 275.4 ng/mL in plasma (EDTA) and 375.6 ng/mL in plasma (heparin).

Protocols

References

ab208349 has not yet been referenced specifically in any publications.

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