Product nameMouse Angiogenin Matched Antibody Pair Kit
See all Angiogenin kits
Assay typeELISA set
Range31.2 pg/ml - 2000 pg/ml
Species reactivityReacts with: Mouse
Matched Antibody Pair kit is a titrated unlabeled capture antibody, a titrated biotin-labeled detector and a calibrated protein standard. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Angiogenin. Both capture and detector antibodies are rabbit monoclonal antibodies.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Additional protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website.
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab208349) which uses the same antibody pair.
To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.
The capture antibody is glycerol free.
The detector antibody contains glycerol.
Tested applicationsSuitable for: ELISAmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 10 x 96 tests 2 x 96 tests Mouse Angiogenin Capture Antibody 1 x 100µg 1 x 20µg Mouse Angiogenin Detector Antibody 1 x 25µg 1 x 5µg Mouse Angiogenin Lyophilized Protein 1 vial 1 vial
FunctionMay function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.
Tissue specificityExpressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons.
Involvement in diseaseDefects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9) [MIM:611895]. ALS is a degenerative disorder of motor neurons in the cortex, brain stem and spinal cord. ALS is characterized by muscular weakness and atrophy.
Sequence similaritiesBelongs to the pancreatic ribonuclease family.
Developmental stageLow level expression in the developing fetus, increased in the neonate, and maximal in the adult.
- Information by UniProt
- AMYOTROPHIC LATERAL SCLEROSIS
SimpleStep ELISA kits
Our Abpromise guarantee covers the use of ab215073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
ab215073 has not yet been referenced specifically in any publications.