Overview

  • Product name
    Mouse Angiopoietin 2 ELISA Kit
    See all Angiopoietin 2 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type
    Cell culture supernatant, Urine, Serum, Heparin Plasma, EDTA Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    156 pg/ml - 10000 pg/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s mouse Angiopoietin-2 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse Angiopoietin-2 in cell culture supernatants, serum and plasma (heparin, EDTA). 


    An Angiopoietin-2 specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Angiopoietin-2 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse Angiopoietin-2 amount of sample captured in plate.


    Get better reproducibility in only 90 minutes with Mouse Angiopoietin 2 ELISA Kit (ab209883) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    ABC Diluent Buffer 1 x 12ml
    Antibody Diluent Buffer 1 x 12ml
    Anti-mouse Angiopoietin-2 Antibody Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-mouse Angiopoietin 2 antibody 1 x 130µl
    Lyophilized recombinant mouse Angiopoietin 2 standard 2 x 10ng
    Plate Seal 1 x 4 units
    Sample Diluent Buffer 1 x 30ml
    TMB Color Developing Agent 1 x 10ml
    TMB Stop Solution 1 x 10ml
  • Research areas
  • Function
    Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
  • Sequence similarities
    Contains 1 fibrinogen C-terminal domain.
  • Domain
    The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • AGPT2
    • ANG 2
    • ANG-2
    • ANG2
    • Angiopoietin 2a
    • Angiopoietin 2B
    • Angiopoietin-2
    • Angiopoietin2
    • ANGP2_HUMAN
    • ANGPT 2
    • Angpt2
    • Tie2 ligand
    see all
  • Database links

    Associated products

    Applications

    Our Abpromise guarantee covers the use of ab171335 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use at an assay dependent concentration.

    Images

    • ab171335 Angiopoietin 2 Mouse ELISA kit
      ab171335 Angiopoietin 2 Mouse ELISA kit
    • ab171335 Angiopoietin 2 Mouse ELISA kit
      ab171335 Angiopoietin 2 Mouse ELISA kit
    • Typical standard curve
      Typical standard curve

      Representative standard curve using ab171335

    Protocols

    References

    This product has been referenced in:
    • Kim J  et al. Heterogeneous perivascular cell coverage affects breast cancer metastasis and response to chemotherapy. JCI Insight 1:e90733 (2016). ELISA ; Human . Read more (PubMed: 28018977) »

    See 1 Publication for this product

    Publishing research using ab171335? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    2 reviews or Q&A

    10
    Votes: Highest First

    I wanted to confirm that running this kit on samples with the Anti-coagulant Heparin would not affect the test?

    Thank you for contacting us. Yes, the Angiopoietin 2 Mouse ELISA kit ab171335 is suitable for assays of plasma collected with heparin.

    Have the antibodies in this ELISA been tested for cross-reactivity with ANG-2, TIE2, and soluble TIE2?

    Recombinant mouse TIE2 at 50ng/ml was tested, and no significant cross-reactivity was observed. There is no detectable cross-reactivity with other relevant proteins such as Ang-1, BDNF and IL-7.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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