Mouse Angiopoietin 2 ELISA Kit (ab171335)

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ab171335 Angiopoietin 2 Mouse ELISA kit
  • ab171335 Angiopoietin 2 Mouse ELISA kit
  • Typical standard curve

Key features and details

  • Sensitivity: 10 pg/ml
  • Range: 156 pg/ml - 10000 pg/ml
  • Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse Angiopoietin 2 ELISA Kit
    See all Angiopoietin 2/ANG2 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 210pg/ml 10.5 = 5%
    2 16 1256pg/ml 55.06 = 4.4%
    3 16 4765pg/ml 274.67 = 5.8%
    Inter-assay
    Sample n Mean SD CV%
    1 24 230pg/ml 11.5 = 5%
    2 24 1185pg/ml 54.51 = 4.6%
    3 24 4889pg/ml 303.11 = 6.2%

  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    < 10 pg/ml

  • Range

    156 pg/ml - 10000 pg/ml

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Abcam’s mouse Angiopoietin-2 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse Angiopoietin-2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). 


    An Angiopoietin-2 specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Angiopoietin-2 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse Angiopoietin-2 amount of sample captured in plate.


    Get better reproducibility in only 90 minutes with Mouse Angiopoietin 2 ELISA Kit (ab209883) from our SimpleStep ELISA® range.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-mouse Angiopoietin-2 Antibody Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl
    Biotinylated anti-mouse Angiopoietin 2 antibody 1 x 100µl
    Lyophilized recombinant mouse Angiopoietin 2 standard 2 x 10ng
    Plate Seal 1 x 4 units
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Amber Bottle 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml

  • Research areas

  • Function

    Can induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.

  • Sequence similarities

    Contains 1 fibrinogen C-terminal domain.

  • Domain

    The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • AGPT 2
    • Agpt2
    • ANG 2
    • ANG-2
    • ANG2
    • Angiopoietin 2a
    • Angiopoietin 2B
    • Angiopoietin-2
    • Angiopoietin2
    • ANGP2_HUMAN
    • ANGPT 2
    • Angpt2
    • Tie2 ligand
    see all

  • Database links

    Associated products

    Images

    • ab171335 Angiopoietin 2 Mouse ELISA kit
      ab171335 Angiopoietin 2 Mouse ELISA kit
    • ab171335 Angiopoietin 2 Mouse ELISA kit
      ab171335 Angiopoietin 2 Mouse ELISA kit
    • Typical standard curve
      Typical standard curve

      Representative standard curve using ab171335

    References (2)

    Publishing research using ab171335? Please let us know so that we can cite the reference in this datasheet.

    ab171335 has been referenced in 2 publications.

    • Kim J  et al. Heterogeneous perivascular cell coverage affects breast cancer metastasis and response to chemotherapy. JCI Insight 1:e90733 (2016). ELISA ; Human . PubMed: 28018977
    • Jenny Zhou H  et al. Endothelial exocytosis of angiopoietin-2 resulting from CCM3 deficiency contributes to cerebral cavernous malformation. Nat Med 22:1033-1042 (2016). PubMed: 27548575

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    I wanted to confirm that running this kit on samples with the Anti-coagulant Heparin would not affect the test?

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    Answer

    Thank you for contacting us. Yes, the Angiopoietin 2 Mouse ELISA kit ab171335 is suitable for assays of plasma collected with heparin.

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    Question

    Have the antibodies in this ELISA been tested for cross-reactivity with ANG-2, TIE2, and soluble TIE2?

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    Answer

    Recombinant mouse TIE2 at 50ng/ml was tested, and no significant cross-reactivity was observed. There is no detectable cross-reactivity with other relevant proteins such as Ang-1, BDNF and IL-7.

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