Overview

  • Product name

    Mouse Annexin A2 ELISA Kit
    See all Annexin-2/ANXA2 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    heart ext 5 3.1%
    Inter-assay
    Sample n Mean SD CV%
    heart ext 3 5.8%
  • Sample type

    Cell culture supernatant, Serum, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    17 pg/ml
  • Range

    78.13 pg/ml - 5000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 97 90% - 101%
    Cell culture extracts 112 109% - 114%
    Tissue Extracts 101 89% - 110%
    Cell culture media 113 106% - 120%
    Heparin Plasma 84 83% - 86%
    Citrate Plasma 87 84% - 89%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Annexin A2 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Annexin A2 protein in mouse serum, plasma, cell culture supernatant, and cell and tissue extract samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material.  TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:
    Sample Diluent NS MDD = 18 pg/mL
    1X Cell Extraction Buffer PTR MDD = 17 pg/mL

  • Notes

    Annexin A2 is a secreted 36 kDa calcium-dependent phospholipid-binding protein localized on the surface of most eukaryotic cells. Annexin A2 is involved in several biological processes, including anti-inflammatory effects, Ca2+-dependent exocytosis, immune responses, Ca2+ transport and phospholipase A2 regulation. Annexin A2 forms a heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of Annexin A2/p36. Annexin A2 binds two calcium ions with high affinity. Annexin A2 inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse Annexin A2 Capture Antibody 1 x 600µl
    10X Mouse Annexin A2 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BR 1 x 6ml
    Mouse Annexin A2 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
  • Sequence similarities

    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • Domain

    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications

    Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
    ISGylated.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
  • Information by UniProt
  • Alternative names

    • Annexin A2
    • Annexin II
    • Annexin II, heavy chain
    • Annexin-2
    • ANX 2
    • ANX2
    • ANX2L4
    • ANXA2
    • ANXA2_HUMAN
    • arylsulfatase B
    • CAL1H
    • Calpactin I heavy chain
    • Calpactin I heavy polypeptide
    • calpactin I heavy polypeptide (p36)
    • Calpactin-1 heavy chain
    • chromobindin 8
    • Chromobindin-8
    • Epididymis secretory protein Li 270
    • HEL S 270
    • LIP2
    • Lipocortin II
    • LPC2
    • LPC2D
    • p36
    • P36 protein
    • PAP-IV
    • Placental anticoagulant protein IV
    • Protein I
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab210576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Annexin A2 were measured in duplicates, interpolated from the Annexin A2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (heparin) 50%.The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • The concentrations of Annexin A2 were measured in duplicate and interpolated from the Annexin A2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Annexin A2 concentration was determined to be 5,627 pg/mL in NIH/3T3 cell extract and 8,735 pg/mL in mouse heart extract samples.

  • The concentrations of Annexin A2 were measured in duplicate and interpolated from the Annexin A2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Annexin A2 concentration was determined to be 6,362 pg/mL in neat mouse lung supernatant sample.

Protocols

References

ab210576 has not yet been referenced specifically in any publications.

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