Overview

  • Product name

    Mouse Annexin A2 Matched Antibody Pair Kit
    See all Annexin-2/ANXA2 kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Range

    62.5 pg/ml - 4000 pg/ml
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Annexin A2.


    Both capture and detector antibodies are rabbit monoclonal antibodies.


    Matched antibody pair kits and reagents deliver consistent, specific, and sensitive results.



    • Batch-to-batch consistency: only recombinant monoclonal antibodies are used in our matched antibody pairs.

    • Specificity: antibody pairs are screened in plasma and serum to ensure specificity in complex samples.

    • Sensitivity: benchmarked against commercially available antibody pairs to ensure equivalent or superior performance compared with the competition.


    Additional buffers and plates are required for the assay. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab210576) which uses the same antibody pair.


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 2 x 96 tests
    Mouse Annexin A2 Capture Antibody 1 x 100µg 1 x 20µg
    Mouse Annexin A2 Detector Antibody 1 x 25µg 1 x 5µg
    Mouse Annexin A2 Lyophilized Protein 1 vial 1 vial
  • Research areas

  • Function

    Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
  • Sequence similarities

    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • Domain

    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications

    Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
    ISGylated.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
  • Information by UniProt
  • Alternative names

    • Annexin A2
    • Annexin II
    • Annexin II, heavy chain
    • Annexin-2
    • ANX 2
    • ANX2
    • ANX2L4
    • ANXA2
    • ANXA2_HUMAN
    • arylsulfatase B
    • CAL1H
    • Calpactin I heavy chain
    • Calpactin I heavy polypeptide
    • calpactin I heavy polypeptide (p36)
    • Calpactin-1 heavy chain
    • chromobindin 8
    • Chromobindin-8
    • Epididymis secretory protein Li 270
    • HEL S 270
    • LIP2
    • Lipocortin II
    • LPC2
    • LPC2D
    • p36
    • P36 protein
    • PAP-IV
    • Placental anticoagulant protein IV
    • Protein I
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab218792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • Standard calibration curve. Background subtracted values are graphed.

Protocols

References

ab218792 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab218792.
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