Mouse Antithrombin III ELISA Kit (SERPINC1) (ab108800)

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Overview

  • Product name

    Mouse Antithrombin III ELISA Kit (SERPINC1)
    See all Antithrombin III kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.4%

  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    = 3 ng/ml

  • Range

    3.13 ng/ml - 100 ng/ml

  • Recovery

    96 %

  • Assay time

    4h 00m

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Abcam’s Antithrombin III (SERPINC1) Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Antithrombin III concentrations in mouse plasma, serum, urine and cell culture supernatants.


    An Antithrombin III specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Antithrombin III specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Antithrombin III captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Sandwich ELISA - Ms anti-thrombine III (ab108800)
    Sandwich ELISA - Ms anti-thrombine III (ab108800)

    Standard curve : mean of duplicates (+/-SD) with backgrounds readings subtracted

  • Sandwich ELISA - Antithrombin III (SERPINC1) Mouse ELISA Kit (ab108800)
    Sandwich ELISA - Antithrombin III (SERPINC1) Mouse ELISA Kit (ab108800)

    Ms antithrombine III measured in biological fluids showing quantity (ng) per mL of sample tested

Protocols

References

This product has been referenced in:

  • Foster DJ  et al. Advanced siRNA Designs Further Improve In Vivo Performance of GalNAc-siRNA Conjugates. Mol Ther 26:708-717 (2018). Read more (PubMed: 29456020) »
See 1 Publication for this product

Publishing research using ab108800? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Question

There is a great deal of variability between lots with the standards, which causes the same samples run with different lots to give different readings.

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Answer

Thank you for your phone call.
The standards for these kits are supplied in different masses. The mass for each vial will be printed on the label, and each lot will require a different volume of diluent in order to generate the 100 ng/mL standard. I hope this helps, please let me know if you need any additional information or assistance.

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Question

biotinylated mouse antibody, lot GR81436-1
vial says 50x
sent protocol says 80x
online protocol says: 50x

we switched from 50x (140 ul) to 80x (100 ul)
ensure updates

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Answer

Thank you for your patience.

Yes, I can confirm that the vial you received is 50x which is correct in this case - for the lot you received. We are looking into updating the datasheet. Going forward 80x will be the correct amount.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

biotinylated mouse antibody, lot GR81436-1
vial says 50x
sent protocol says 80x
online protocol says: 50x

we switched from 50x (140 ul) to 80x (100 ul)
ensure updates

Read More
Answer

Thank you for contacting us.

I have contacted the corresponding department regarding your inquiry and hope to have an answer for you soon. I will be in touch.

I am sorryfor the confusion, and thank you for your patience.

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Question

I have been able to successfully plot the standard curve. See attached file. I tested serum and citrated plasma from naïve male CD1 mice, and obtained AT3 levels in the range of 400-500 ug/ml (after factoring in the 1:16,000 dilution). Are these values normal?  

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Answer

Thank you for contacting us. I have had a chance to speak with the lab regarding your question. Our data suggested that mouse serum ATIII has slightly higher concentration than plasma. We have about 200 – 600 ug/ml range of different mouse samples. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

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Question

What are the expected baseline levels in serum?

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Answer

Thank you for contacting us. I was hoping that you might fill me in on a couple of points about your results with this assay. Have you been able to plot the standard curve accurately using the mean value of the triplicate readings for each standard and sample?  The minimum detectable dose of mATIII is typically 1ng/ml. What readings are you seeing with your sample?   Thank you in advance for getting this information for me. Please do not hesitate to contact us if you need any more advice or information.  

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