Overview

  • Product name

    Mouse Apo B ELISA Kit
    See all Apolipoprotein B kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 2.4%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 3.9%
  • Sample type

    Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    196 pg/ml
  • Range

    1.56 ng/ml - 100 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 83 75% - 95%
    Cell culture media 104 99% - 107%
    Heparin Plasma 98 91% - 107%
    EDTA Plasma 88 79% - 98%
    Citrate Plasma 86 75% - 99%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Human
  • Product overview

    ApoB in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of mouse ApoB protein in serum, plasmas, and cell culture supernatant.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Apolipoprotein B (ApoB) is a 550 kDa member of the large lipid transfer (LLT) superfamily. It is a major protein component of chylomicron, very low-density lipoprotein (VLDL), and low-density lipoprotein (LDL) particles. Apolipoprotein based particles function to transport cholesterol and triglycerides in the circulation.  Circulating ApoB is a critical factor in cardiovascular disease, insulin resistance, and metabolic syndrome. ApoB levels are relatively stable compared to LDL and VLDL levels which rise following food intake. Dietary lipids are absorbed in the small intestine by enterocytes which package them with ApoB into chylomicrons for release into the circulation. Endogenous lipids synthesized in the liver are packaged with ApoB into VLDL particles and released into the serum. ApoB is required for the assembly and secretion of VLDL particles and chylomicrons. Mouse shares 88.7 % and 69.9% sequence homology with rat and human ApoB, respectively.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab230932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of ApoB were measured in duplicate, interpolated from the mouse ApoB standard curve and corrected for sample dilution. Undiluted samples are as follows: serum 1:2000, plasma (citrate) 1:2000, plasma (EDTA) 1:2000, and plasma (heparin) 1:2000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ApoB concentration was determined to be 187.00 µg/mL in serum, 114.525 µg/mL in plasma (citrate) and 149.723 µg/mL in plasma (EDTA), and 152.208 µg/mL in plasma (Heparin).

Protocols

References

ab230932 has not yet been referenced specifically in any publications.

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