Overview

  • Product name

    Mouse APOA1 ELISA Kit
    See all Apolipoprotein A I kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 3 4.7%
    Inter-assay
    Sample n Mean SD CV%
    Serum 8 5.6%
  • Sample type

    Cell culture supernatant, Serum, Tissue Extracts, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    11.22 pg/ml
  • Range

    117.19 pg/ml - 9000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 91 81% - 101%
    Serum 82 79% - 84%
    Tissue Extracts 90 83% - 93%
    Heparin Plasma 92 88% - 96%
    EDTA Plasma 88 85% - 90%
    Citrate Plasma 99 91% - 101%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Rat, Human
  • Product overview

    APOA1 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of APOA1 protein in mouse serum, plasma, cell culture supernatant, and cell extracts.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm. 


    Apolipoprotein AI (ApoA-I) is secreted by the liver and small intestine and is a major protein of plasma HDL (high density lipoprotein). APOA-I participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). Defects in APOA-I are associated with several diseases associated with low HDL levels (HDLD1 and HDLD2) and amyloidosis (AMYLIOWA and AMYL8). Human and Rat share 64 and 68.6% sequence homology with Mouse Apolipoprotein A1, respectively.


    Sensitivity
    Samples Diluted in sample Diluent NS - 11.22 pg/mL
    Sample Diluted in 1X Cell Extraction Buffer PTR - 20.04 pg/mL

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab238260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • The APOA1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The APOA1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
  • The concentrations of APOA1 were measured in duplicates, interpolated from the APOA1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:50,000, plasma (citrate) 1:25,000, plasma (EDTA) 1:25,000, and plasma (heparin) 1:50,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean APOA1 concentration was determined to be 81.08 ug/mL in serum, 42.54 ug/mL in plasma (citrate), 70.10 ug/mL in plasma (EDTA), and 86.01 ug/mL in plasma (Heparin).

  • The concentrations of APOA1 were measured in duplicates, interpolated from the APOA1 standard curves and corrected for sample dilution. Undiluted sample was liver supernatant 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean APOA1 concentration was determined to be 110.43 ng/mL in liver supernatant.
  • The concentrations of APOA1 were measured in duplicate and interpolated from the APOA1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean APOA1 concentration was determined to be 5.49 ng/mL.

Protocols

References

ab238260 has not yet been referenced specifically in any publications.

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