Overview

  • Product name

    Mouse Axl ELISA Kit
    See all Axl kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 2.4%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 3.4%
  • Sample type

    Cell culture supernatant, Urine, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    10.8 pg/ml
  • Range

    62.5 pg/ml - 4000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 106 101% - 114%
    Urine 109 104% - 114%
    Serum 104 91% - 116%
    Heparin Plasma 83 74% - 91%
    EDTA Plasma 100 86% - 111%
    Citrate Plasma 102 90% - 111%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Rabbit, Cow, Dog, Human, Pig
  • Product overview

    Abcam’s Axl in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Axl protein in serum, plasma, urine and cell culture supernatant.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Axl is a transmembrane receptor tyrosine kinase on the plasma membrane. The growth factor GAS6 is a ligand for Axl and regulates many physiological processes including cell survival, cell proliferation, migration and differentiation. The antibodies used in this kit were raised against a portion of the extracellular domain of Axl.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab207619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Axl were measured in duplicates, interpolated from the Axl standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 4%, plasma (citrate) 4%, plasma (EDTA) 4%, and urine 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Axl concentration was determined to be 113 ng/mL in serum, 73.3 ng/mL in plasma (citrate), 88.9 ng/mL in plasma (heparin), and 112 ng/mL in urine.

  • L929 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. Axl was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated L929 and cell culture media. Measured values were interpolated from the Axl Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 169 pg/mL unstimulated, 696 pg/mL stimulated, and undetectable in media.

Protocols

References

ab207619 has not yet been referenced specifically in any publications.

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