Mouse Bax ELISA Kit (ab233624)
- Datasheet
- References
- Protocols
Overview
-
Product name
Mouse Bax ELISA Kit
See all Bax kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% cell extract 5 8.1% Inter-assay Sample n Mean SD CV% cell extract 3 6.3% -
Sample type
Cell Lysate, Tissue Homogenate -
Assay type
Sandwich (qualitative) -
Sensitivity
19.4 pg/ml -
Range
46.9 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 101 97% - 108% -
Assay time
2h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Bax in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Bax protein in mouse cell lysates and tissue homogenate.
The ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Bax, also known as Apoptosis Regulator BAX, is a pro-apoptotic member of Bcl-2 protein family. Bax accelerates cell death by binding to and antagonizing the apoptosis repressor, Bcl-2. It also undergoes a translocation to the mitochondrion membrane during cell stress, leading to the release of cytochrome c which then triggers apoptosis.
-
Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 1 x 96 tests 10X Mouse 10X Detector Antibody 1 x 600µl 1 x 600µl 10X Mouse Bax Capture Antibody 1 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 2 x 20ml 2 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml 1 x 10ml Antibody Diluent 4BR 1 x 6ml 1 x 6ml Mouse Bax Lyophilized Recombinant Protein 2 vials 2 vials Plate Seals 1 unit 1 unit Sample Diluent NS (ab193972) 1 x 50ml 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 1 unit Stop Solution 1 x 12ml 1 x 12ml Streptavidin-HRP 2 x 100µl 2 x 100µl TMB Development Solution 1 x 12ml 1 x 12ml -
Research areas
-
Function
Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. -
Tissue specificity
Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. -
Sequence similarities
Belongs to the Bcl-2 family. -
Domain
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. -
Cellular localization
Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane. - Information by UniProt
-
Alternative names
- Apoptosis regulator BAX
- BAX
- Bax-protein
see all -
Database links
- Entrez Gene: 12028 Mouse
- SwissProt: Q07813 Mouse
- Unigene: 19904 Mouse
Associated products
-
Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab233624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
-
Example of mouse Bax standard curve in 1X Cell Extraction Buffer PTR.The Bax standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native Bax in mouse cell extracts.Interpolated concentrations of native Bax in mouse C2C12 cell extract based on a 12.5 µg/mL extract load, A20 cell extract based on a 5 µg/mL extract load, and PC-12 cell extract based on a 25 µg/mL extract load. The concentrations of Bax were measured in duplicate and interpolated from the Bax standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Bax concentration was determined to be 3,001 pg/mL in C2C12 cell extract, 1,404 pg/mL in A20 cell extract, and 1,689 pg/mL in PC-12 cell extract.
-
Comparison of Bax distribution in subcellular fractions derived from 3.7x103 HeLa cells and whole cells cultured in the presence (treated) or absence (untreated) of 1 µM staurosporine for 4 hours.Cells were collected directly after treatment and subcellular fractions were prepared using a cell fractionation kit (ab109719). The concentrations of Bax were measured in three different dilutions of the fraction samples in duplicate and interpolated from the Bax standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean Bax concentration was determined to be 841.4 pg/mL in the untreated cytosol fraction, 100.0 pg/mL in the untreated mitochondrial fraction, 912.6 pg/mL in the untreated whole cell sample, 186.9 pg/mL in the treated cytosol fraction, 1,366 pg/mL in the treated mitochondrial fraction, and 1,622 pg/mL in the treated whole cell sample.
-
Other species reactivity was determined by measuring Bax in rat and human cell extracts within the working range of the assay, interpolating the protein concentrations from the mouse standard curve.The mean Bax concentration was determined to be 243.5 ng/mg in C2C12 cell extract, 65.15 ng/mg in PC-12 cell extract, and 39.85 ng/mg in HeLa cell extract.
Protocols
References
ab233624 has not yet been referenced specifically in any publications.
