Overview

  • Product name

    Mouse BCA1 ELISA Kit
    See all BCA1 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 2.1%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 5.5%
  • Sample type

    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    4.1 pg/ml
  • Range

    12.5 pg/ml - 800 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 82 76% - 87%
    Serum 116 112% - 118%
    Heparin Plasma 84 78% - 94%
    EDTA Plasma 126 118% - 135%
    Citrate Plasma 102 102% - 103%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Cow, Human
  • Product overview

    BCA1 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of BCA1 protein in mouse serum, plasma and cell culture supernatant.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material.  TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Mouse B-lymphocyte chemoattractant/B Cell-attracting Chemokine 1 (BCA1/CXCL13) is a member of the CXC chemokine superfamily. BCA1 (CXCL13) is produced by stromal cells from the liver, spleen, lymph nodes and the gut. It binds to the CXCR5 receptor in lymphoid follicles controlling the homing signal of B cells and subsets of T cells to the lymphoid nodes, compartmentalizing lymphocytes and antigen presenting cells within the follicles of lymphoid tissues. BCA-1 (CXCL13) is also required for the embryonic development of most of lymph nodes and Peyer’s patches. CXCL13-/- as well as CXCR5-/- mice lack secondary lymphoid structures and show a disrupted splenic architecture.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse BCA1 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BR 1 x 6ml
    Mouse BCA1 Capture Antibody (lyophilized) 1 vial
    Mouse BCA1 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5.
  • Tissue specificity

    Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • ANGIE
    • ANGIE2
    • B cell attracting chemokine 1
    • B cell-attracting chemokine 1
    • B lymphocyte chemoattractant
    • B-cell chemoattractant
    • B-cell-attracting chemokine 1
    • B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1)
    • BCA-1
    • BLC
    • BLR1L
    • C-X-C motif chemokine 13
    • Chemokine (C-X-C motif) ligand 13
    • Chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant)
    • Chemokine, CXC motif, ligand 13
    • CXC chemokine BLC
    • CXCL13
    • CXL13_HUMAN
    • SCYB13
    • Small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant)
    • Small inducible cytokine B13
    • Small inducible cytokine subfamily B, member 13
    • Small-inducible cytokine B13
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab212167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of BCA1 were measured in duplicates, interpolated from the BCA1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (EDTA) 25%, plasma (citrate) 25%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BCA1 concentration was determined to be 580 pg/mL in serum, 377pg/mL in plasma (EDTA), 282 pg/mL in plasma (citrate) and 2,884 pg/mL in plasma (heparin).

  • The concentrations of BCA1 were measured in duplicates, interpolated from the BCA1 standard curves and corrected for sample dilution. Undiluted samples are as follows: lung cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BCA1 concentration was determined to be 348 pg/mL in lung cell culture supernatant.

Protocols

References

ab212167 has not yet been referenced specifically in any publications.

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