Overview

  • Product name

    Mouse BCA1 Matched Antibody Pair Kit
    See all BCA1 kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Range

    31.25 pg/ml - 2000 pg/ml
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Matched Antibody Pair kit is a titrated unlabeled capture antibody, a titrated biotin-labeled detector and a calibrated protein standard. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse BCA1.


    Both capture and detector antibodies are rabbit monoclonal antibodies.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Additional protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website.


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab212167) which uses the same antibody pair.


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.


    Buffer information:
    The capture antibody is glycerol free.
    The detector antibody contains glycerol.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 2 x 96 tests
    Mouse BCA1 Capture Antibody 1 x 100µg 1 x 20µg
    Mouse BCA1 Detector Antibody 1 x 25µg 1 x 5µg
    Mouse BCA1 Lyophilized Protein 1 vial 1 vial
  • Research areas

  • Function

    Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5.
  • Tissue specificity

    Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • ANGIE
    • ANGIE2
    • B cell attracting chemokine 1
    • B cell-attracting chemokine 1
    • B lymphocyte chemoattractant
    • B-cell chemoattractant
    • B-cell-attracting chemokine 1
    • B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1)
    • BCA-1
    • BLC
    • BLR1L
    • C-X-C motif chemokine 13
    • Chemokine (C-X-C motif) ligand 13
    • Chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant)
    • Chemokine, CXC motif, ligand 13
    • CXC chemokine BLC
    • CXCL13
    • CXL13_HUMAN
    • SCYB13
    • Small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant)
    • Small inducible cytokine B13
    • Small inducible cytokine subfamily B, member 13
    • Small-inducible cytokine B13
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab218172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • Standard calibration curve. Background subtracted values are graphed.

Protocols

References

ab218172 has not yet been referenced specifically in any publications.

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