Overview

  • Product name

    Mouse Beta-2-Microglobulin ELISA Kit
    See all beta 2 Microglobulin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 2.8%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 3.2%
  • Sample type

    Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.165 pg/ml
  • Range

    3.125 pg/ml - 200 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 114 110% - 118%
    Urine 98 95% - 100%
    Serum 102 97% - 106%
    Hep Plasma 93 82% - 103%
    EDTA Plasma 103 101% - 107%
    Cit plasma 102 101% - 103%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Beta-2-Microglobulin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Beta-2-Microglobulin protein in mouse serum, plasma, urine and cell culture supernatant samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Beta-2-Microglobulin is the light chain of the class I major histocompatibility complex (MHC); it forms heterodimers with classical (HLA-A, -B, -C) and non-classical (HLA-E, -F, -G) class I MHC heavy chains and with class I non-MHC heavy chains (CD1). Beta-2-Microglobulin is involved in the presentation of peptide antigens to the immune system. Beta-2-Microglobulin can dissociate from the MHC and circulate in complex with alpha 2-Macroglobulin.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 5BR 1 x 6ml
    Denaturant 1 x 500µl
    10X Mouse Beta-2-Microglobulin Capture Antibody 1 x 600µl
    10X Mouse Beta-2-Microglobulin Detector Antibody 1 x 600µl
    Mouse Beta-2-Microglobulin Lyophilized Recombinant Protein 1 x 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
  • Involvement in disease

    Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
    Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.
  • Sequence similarities

    Belongs to the beta-2-microglobulin family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    Glycation of Ile-21 is observed in long-term hemodialysis patients.
  • Cellular localization

    Secreted. Detected in serum and urine.
  • Information by UniProt
  • Alternative names

    • B2M
    • B2MG_HUMAN
    • Beta 2 microglobin
    • Beta 2 microglobulin
    • Beta 2 microglobulin precursor
    • Beta chain of mhc class 1 proteins
    • Beta chain of MHC class I molecules
    • Beta-2-microglobulin form pI 5.3
    • CDABP0092
    • Hdcma22p
    • IMD43
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab223590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Beta-2-Microglobulin were measured in duplicates, interpolated from the Beta-2-Microglobulin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:100,000, plasma (citrate) 1:100,000, plasma (heparin) 1:100,000, and plasma (EDTA) 1:100,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Beta-2-Microglobulin concentration was determined to be 19.48 µg/mL in neat serum, 15.15 µg/mL in neat plasma (citrate), 18.74 µg/mL in neat plasma (heparin), and 15.87 µg/mL in neat plasma (EDTA).

  • The concentrations of Beta-2-Microglobulin were measured in duplicates, interpolated from the Beta-2-Microglobulin standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 1:100, lung supernatant 1:5,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Beta-2-Microglobulin concentration was determined to be 16.12 ng/mL in neat urine, 440.7 ng/mL in neat lung tissue culture supernatant.

Protocols

References

ab223590 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Abreviews
We have used the ab223590 ELISA Kit for measurement of urine b-2-MIG in a GN mouse modell. The Kit is easy to use and the assay is quickly prepared.
However, the recommended dilutions for mouse urine (1:100 - 1:1600) were too low for our samples. We had to dilute our samples further. Dilutions from 1:2000 - 1:10000 worked for most samples, but others were still too concentrated. So my advice for future usage would be to test a wide variety of dilutions (at least for urine samples).

Abcam user community

Verified customer

Submitted Jul 15 2019

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