Key features and details
- Sensitivity: 3 pg/ml
- Range: 3 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse beta IGH3 ELISA Kit
See all IGH3 kits
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range3 pg/ml - 1000 pg/ml
Sample specific recovery Sample type Average % Range Serum 140.1 132% - 148% Plasma 136 115% - 150% Cell culture media 143.6 137% - 149%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Abcam’s beta IGH3 mouse ELISA Kit (ab193717) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse BIGH3 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse beta IGH3 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures beta IGH3 present in the samples. The wells are washed and biotinylated anti-mouse beta IGH3 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of beta IGH3 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated mouse beta-IGH3 detection antibody (lyophilized) 2 vials Mouse beta-IGH3 standards (lyophilized) 2 vials Pre-coated mouse beta-IGH3 Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
Cellular localizationSecreted, extracellular space, extracellular matrix.
- Beta ig-h3
SimpleStep ELISA kits
ab193717 has not yet been referenced specifically in any publications.