Overview

  • Product name

    Mouse beta IGH3 ELISA Kit
    See all IGH3 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    3 pg/ml
  • Range

    3 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 140.1 132% - 148%
    Plasma 136 115% - 150%
    Cell culture media 143.6 137% - 149%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s beta IGH3 mouse ELISA Kit (ab193717) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse BIGH3 in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for mouse beta IGH3 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures beta IGH3 present in the samples. The wells are washed and biotinylated anti-mouse beta IGH3 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of beta IGH3 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get better reproducibility in only 90 minutes with Mouse beta IG H3 ELISA Kit (ab206987) from our SimpleStep ELISA® range.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab193717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Typical Standard Curve using ab193717 BIGH3 Mouse ELISA Kit.

Protocols

References

ab193717 has not yet been referenced specifically in any publications.

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