Key features and details
- Sensitivity: 40 pg/ml
- Range: 0.041 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse Beta nerve growth factor ELISA Kit
See all Beta nerve growth factor kits
Intra-assay Sample n Mean SD CV% General < 10% Inter-assay Sample n Mean SD CV% General < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range0.041 ng/ml - 100 ng/ml
Sample specific recovery Sample type Average % Range Serum 117.8 81% - 149% Plasma 108.8 78% - 148% Cell culture media 108.4 79% - 139%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Predicted to work with: Human
Mouse Beta nerve growth factor ELISA Kit (ab277398) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Beta nerve growth factor in serum (mouse beta-NGF concentration is low in normal serum and plasma samples, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse Beta nerve growth factor coated on a 96-well plate. Standards and samples are pipetted into the wells and Mouse Beta nerve growth factor present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Mouse Beta nerve growth factor antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Mouse Beta nerve growth factor bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Tested applicationsSuitable for: Sandwich ELISAmore details
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated Anti-Mouse Beta nerve growth factor Antibody 2 vials Anti-Mouse Beta nerve growth factor coated Microplate 1 unit Mouse Beta nerve growth factor standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
RelevanceNerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture, NGF induces the formation of neurite projections and, in vivo, may stimulate the innervation of tissues. NGF plays a role in the repair, regeneration, and protection of neurons, and as such could serve as a therapeutic agent in neurodegenerative conditions such as Alzheimer's disease. NGF enhances survival, growth, neurotransmitter biosynthesis of sympathetic and sensory neurons; neurotrophic factor; cutaneous innervation; growth, differentiation and survival of B lymphocytes. It also has a possible role in allergy and tissue repair. NGF is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. NGF is upregulated by glutamate, vitamin D3, IL6, FGF basic, astrocyte specific IL1, TNF alpha, PDGF and TGF beta. It is downregulated by GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta.
- Beta nerve growth factor
- Beta NGF
Our Abpromise guarantee covers the use of ab277398 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab277398 has not yet been referenced specifically in any publications.