Mouse Betacellulin Antibody Pair - BSA and Azide free (BTC) (ab256757)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Mouse
Overview
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Product name
Mouse Betacellulin Antibody Pair - BSA and Azide free (BTC)
See all BTC kits -
Assay type
ELISA set -
Range
4.69 pg/ml - 300 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
The Antibody Pair can be used to quantify Mouse Betacellulin (BTC). BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab255722), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Mouse Betacellulin (BTC) Capture Antibody (unconjugated) 1 x 100µg Mouse Betacellulin (BTC) Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. The effects of betacellulin are probably mediated by the EGF receptor and other related receptors. -
Tissue specificity
Synthesized in several tissues and tumor cells. Predominantly expressed in pancreas and small intestine. -
Sequence similarities
Contains 1 EGF-like domain. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
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Alternative names
- Betacellulin
- Betacellulin precursor
- BTC
see all -
Database links
- Entrez Gene: 12223 Mouse
- SwissProt: Q05928 Mouse
- Unigene: 2024 Mouse
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab256757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| Notes |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
Images
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Sandwich ELISA - Mouse Betacellulin Antibody Pair - BSA and Azide free (BTC) (ab256757)Representative standard curve from corresponding SimpleStep ELISA® Kit (ab255722), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.
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Sandwich ELISA - Mouse Betacellulin Antibody Pair - BSA and Azide free (BTC) (ab256757)To learn more about the advantages of recombinant antibodies see here.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab256757 has not yet been referenced specifically in any publications.
