Mouse brain tissue lysate - mitochondrial extract (ab110345)

Overview

  • Product name
    Mouse brain tissue lysate - mitochondrial extract
    See all Brain lysates
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

    50 µg of mouse brain mitochondria solubilized in SDS-PAGE loading buffer and supplemented with reducing agent. This sample is ready for SDS-electrophoresis and acts as a positive control in Western blotting applications.

  • Tested applications
    Suitable for: SDS-PAGE, WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    Constituent: 99% SDS-PAGE Sample Buffer
  • Concentration information loading...
  • Lysate notes
    50 µg of mouse brain mitochondria solubilized in SDS-PAGE loading buffer and supplemented with reducing agent. This sample is ready for SDS-electrophoresis and acts as a positive control in Western blotting applications.

Applications

Our Abpromise guarantee covers the use of ab110345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
SDS-PAGE Use at an assay dependent dilution.
WB Use at an assay dependent dilution. 10.0 µg/lane

References

ab110345 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer



As we discussed over the phone, I have contacted the lab at Mitosciences to get their input into this query. The mouse brain mitochondrial lysate has been prepared using standard mitochondrial isolation procedures. These procedures separate the mitochondria based on differential centrifugation - mitochondria pellet at 12000g after having removed larger cellular components. As such they are highly enriched for mitochondria but not pure. The quality control of the lysate has been performed using mitochondrial antibody cocktails, please see the image attached. This is true of the kit ab110168 as well.

To make an assessment of purity one would need to look at a variety of non mitochondrial components to determine the level of contamination. So in short if you are looking for guaranteed highly pure mitochondria (for example >90%) we wouldn’t recommend these products.

Essentially very pure mitochondiral fractions are best prepared by taking crude mitos and spinning over a gradient (traditionally percoll, metrizamide sometimes used too). This is not a very scalable procedure so not practical for us to do.

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