Product nameMouse brain tissue lysate - total protein (0 days)
See all Brain lysates
DescriptionMouse brain total protein lysate
Source: Outbred NIH-Swiss Albino mice
The mouse brain tissue was collected from zero (0) day-old mice. The lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris -HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris -HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: 5% Beta mercaptoethanol
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Our Abpromise guarantee covers the use of ab7188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Ready to load on SDS-PAGE for Western blotting, 10-20 µg per lane is recommended for mini gel.|
Anti-Doublecortin antibody (ab18723) at 1 µg +
Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Observed band size: 49 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Jennings JS et al. Circulating ghrelin and leptin concentrations and growth hormone secretagogue receptor abundance in liver, muscle, and adipose tissue of beef cattle exhibiting differences in composition of gain. J Anim Sci 89:3954-72 (2011). WB . Read more (PubMed: 21724941) »