Mouse brain tissue lysate - total protein (ab30151)

Q&A (5)

Overview

  • Product name
    Mouse brain tissue lysate - total protein
    See all Brain lysates
  • Description
    Mouse brain total protein lysate
  • General notes


    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.

  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Store at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • Storage buffer
    Preservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Protease inhibitor, Sodium chloride, EDTA, Magnesium chloride, HEPES, 0.15M Beta mercaptoethanol
  • Concentration information loading...
  • Lysate notes
    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab30151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

References

ab30151 has not yet been referenced specifically in any publications.

Publishing research using ab30151? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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5 Q&A

10
Votes: Highest First

looking for these products in mice 6 weeks or older, are these lysates from adults? or are you able to produce this?

Thank you for contacting us. These two products are supplied as adult lysates, all older than 6 weeks , so they should be suitable for your studies.

I hope this information is helpful to you. Please do not hesitate to contact us if you need an...

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Thank you for your helpful advice. I used Ponceau S on my most
recent blot and was able to visualize transfer of both my MW marker
and proteins in my cell lysate. It's certainly possible, however,
I could be getting over-transfe...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for your suggestions. In reviewing my previous blots, I
realized that all the ones I had run with the commercial cell lysate
had been stained with Amido Black, which made them unsuitable for
stripping and re-probing. I conseque...

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Thank you for your reply.


I have a few additional suggestions. I am not sure if my colleague had mentioned those to you:

1) You can check the transfer with Ponceau S (which is a reversible membrane stain) to be sure that you tr...

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WB with human samples
sees lots of weak bands between 100 and 150 kDa and below 100 kDa
not sure if potein is expressed in samples
had used Ab from other company which gave inconsistent results
loaded 30 ug protein
Ab: 1/500

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Thank you for contacting us.
I am sorry to hear that the antibody might not be working as expected.

As we discussed over the phone, I would recommend the following positive control:

ab30151 (https://www.abcam.com/brain-mouse-wh...

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Do you guys have a positive control for Anti-Cdk2 antibody (ab6538)? I would like to purchase this antibody with a tested positive control. Thanks! sincerely,

Thank you for contacting us. I presume you are looking for positive control used in western blot. Our customers have used this ab successfully with mouse brain tissue and 293 cells lysates. Please check the Abreviews. The Mouse brain lysates ca...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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