Mouse C Reactive Protein ELISA Kit (ab222511)
- Datasheet
- References
- Protocols
Overview
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Product name
Mouse C Reactive Protein ELISA Kit
See all C Reactive Protein kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 2.4% Inter-assay Sample n Mean SD CV% Serum 3 2.1% -
Sample type
Serum, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
31 pg/ml -
Range
156.25 pg/ml - 10000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 111 109% - 113% Tissue Extracts 115 103% - 122% Hep Plasma 113 110% - 117% EDTA Plasma 107 103% - 109% Cit plasma 108 90% - 117% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
C Reactive Protein in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of C Reactive Protein in mouse serum, plasma, and cell and tissue extract samples.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
C Reactive Protein displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. C Reactive Protein can interact with DNA and histones and it may scavenge nuclear material released from damaged circulating cells. C Reactive Protein is secreted; it forms a homopentamer pentaxin (or pentraxin) which have a discoid arrangement of 5 non-covalently bound subunits. C Reactive Protein binds 2 calcium ions per subunit. The concentration of C Reactive Protein in plasma increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL1/interleukin-1and IL6//interleukin-6.
Sensitivity:
Sample diluted in Diluent NS + 1X Enhancer - 31 pg/mL
Sample diluted in 1X Cell Extraction Buffer PTR - 37.4 pg/mL
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse C Reactive Protein Capture Antibody (Lyophilized) 1 vial Mouse C Reactive Protein Detector Antibody (Lyophilized) 1 vial Mouse C Reactive Protein Lyophilized Recombinant Protein 1 x 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. -
Tissue specificity
Found in plasma. -
Sequence similarities
Belongs to the pentaxin family.
Contains 1 pentaxin domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Pentraxin 1, short
- C reactive protein
- C reactive protein pentraxin related
see all -
Database links
- Entrez Gene: 12944 Mouse
- SwissProt: P14847 Mouse
- Unigene: 28767 Mouse
Applications
Our Abpromise guarantee covers the use of ab222511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Other - Mouse C Reactive Protein ELISA Kit (ab222511)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse C Reactive Protein standard curve in 1X Cell Extraction Buffer PTR.The C Reactive Protein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse C Reactivte Protein standard curve in Sample Diluent NS + Enhancer.The C Reactive Protein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native C Reactive Protein in mouse serum and plasma samples.The concentrations of C Reactive Protein were measured in duplicates, interpolated from the C Reactive Protein standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:400, plasma (citrate) 1:800, plasma (heparin) 1:400, and plasma (EDTA) 1:1600. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean C Reactive Protein concentration was determined to be 2854 ng/mL in neat serum, 5469 ng/mL in neat plasma (citrate), 3358 ng/mL in neat plasma (heparin), and 7251 ng/mL in neat plasma (EDTA).
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Interpolated concentrations of native C Reactive Protein in mouse liver tissue extract.Based on a 200 μg/mL extract load and mouse heart tissue extract based on a 500 μg/mL extract load. The concentrations of C Reactive Protein were measured in duplicate and interpolated from the C Reactive Protein standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean C Reactive Protein concentration was determined to be 7416 pg/mL in mouse liver tissue extract and 5978 pg/mL in mouse heart tissue extract.
Protocols
References
ab222511 has not yet been referenced specifically in any publications.
