Mouse C Reactive Protein ELISA Kit (ab157712)

Product name

Mouse C Reactive Protein ELISA Kit (PTX1)
See all C Reactive Protein kits
Detection method

Colorimetric

Precision

Intra-assay
Sample	n	Mean	SD	CV%
Overall				< 10%

Inter-assay
Sample	n	Mean	SD	CV%
Overall				< 10%

Recovery

Sample specific recovery
Sample type	Average %	Range
Serum	> 85	% - %

Assay duration

Multiple steps standard assay
Species reactivity

Reacts with: Mouse
Product overview

Abcam's C Reactive Protein (PTX1) Mouse ELISA kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring C Reactive Protein in mouse biological samples.

In this assay the C Reactive Protein present in samples reacts with the anti-C Reactive Protein antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-C Reactive Protein antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound C Reactive Protein. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of C Reactive Protein in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of C Reactive Protein in the test sample. The quantity of C Reactive Protein in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Tested applications

Suitable for: Sandwich ELISAmore details
Platform

Microplate

Components	1 x 96 tests
100X HRP-conjugated anti-mouse C Reactive Protein antibody	1 x 150µl
20X Wash Buffer Concentrate	1 x 50ml
5X Diluent Concentrate	1 x 50ml
Chromogen Substrate Solution	1 x 12ml
Mouse C Reactive Protein Calibrator (Lyophilized)	1 vial
Mouse C Reactive Protein ELISA Microplate	1 unit
Stop Solution	1 x 12ml

Research areas
Function

Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
Tissue specificity

Found in plasma.
Sequence similarities

Belongs to the pentaxin family.
Contains 1 pentaxin domain.
Cellular localization

Secreted.
Target information above from: UniProt accession P02741 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Alternative names
- Pentraxin 1, short
- C reactive protein
- C reactive protein pentraxin related
- C-reactive protein(1-205)
- CRP
- CRP_HUMAN
- MGC88244
- Pentraxin 1
- PTX 1
- PTX1
see all
Database links
- Entrez Gene: 12944 Mouse
- SwissProt: P14847 Mouse
- Unigene: 28767 Mouse

Our Abpromise guarantee covers the use of ab157712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Sandwich ELISA		Use at an assay dependent concentration.

Standard Curve

Representative standard curve using ab157712 C Reactive Protein Mouse ELISA kit

Pogue AI et al. Systemic Inflammation in C57BL/6J Mice Receiving Dietary Aluminum Sulfate; Up-Regulation of the Pro-Inflammatory Cytokines IL-6 and TNFa, C-Reactive Protein (CRP) and miRNA-146a in Blood Serum. J Alzheimers Dis Parkinsonism 7:N/A (2017). Read more (PubMed: 29354323) »
Schafer S et al. IL-11 is a crucial determinant of cardiovascular fibrosis. Nature 552:110-115 (2017). ELISA . Read more (PubMed: 29160304) »

Publishing research using ab157712? Please let us know so that we can cite the reference in this datasheet.

Mouse C Reactive Protein ELISA Kit (PTX1) (ab157712)