Overview

  • Product name
    Mouse C Reactive Protein ELISA Kit (PTX1)
    See all C Reactive Protein kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type
    Urine, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.169 ng/ml
  • Range
    0.78 ng/ml - 25 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 % - %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam's C Reactive Protein (PTX1) Mouse ELISA kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring C Reactive Protein in mouse biological samples. 

     

    In this assay the C Reactive Protein present in samples reacts with the anti-C Reactive Protein antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-C Reactive Protein antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound C Reactive Protein. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of C Reactive Protein in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of C Reactive Protein in the test sample. The quantity of C Reactive Protein in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab157712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab157712 C Reactive Protein Mouse ELISA kit

Protocols

References

This product has been referenced in:
  • Momtazi-Borojeni AA  et al. A simple and rapid-acting approach for the reduction of C-reactive protein. Biomed Pharmacother 109:2305-2308 (2019). Read more (PubMed: 30551488) »
  • Wang D  et al. Caspse-11-GSDMD pathway is required for serum ferritin secretion in sepsis. Clin Immunol N/A:N/A (2019). Read more (PubMed: 30731209) »
See all 5 Publications for this product

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