Mouse C Reactive Protein ELISA Kit (PTX1) (ab157712)

Abcam's C Reactive Protein (PTX1) Mouse ELISA kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring C Reactive Protein in mouse biological samples. 

In this assay the C Reactive Protein present in samples reacts with the anti-C Reactive Protein antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-C Reactive Protein antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound C Reactive Protein. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of C Reactive Protein in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of C Reactive Protein in the test sample. The quantity of C Reactive Protein in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

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