Key features and details
- Sensitivity: 0.1691 ng/ml
- Range: 0.78 ng/ml - 25 ng/ml
- Sample type: Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse C Reactive Protein ELISA Kit (PTX1)
See all C Reactive Protein kits
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 10%
Sample typeUrine, Serum, Plasma
Assay typeSandwich (quantitative)
Range0.78 ng/ml - 25 ng/ml
Sample specific recovery Sample type Average % Range Serum > 85 % - %
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Abcam's C Reactive Protein (PTX1) Mouse ELISA kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring C Reactive Protein in mouse biological samples.
In this assay the C Reactive Protein present in samples reacts with the anti-C Reactive Protein antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-C Reactive Protein antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound C Reactive Protein. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of C Reactive Protein in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of C Reactive Protein in the test sample. The quantity of C Reactive Protein in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 100X HRP-conjugated anti-mouse C Reactive Protein antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml Mouse C Reactive Protein Calibrator (Lyophilized) 1 vial Mouse C Reactive Protein ELISA Microplate 1 unit Stop Solution 1 x 12ml
FunctionDisplays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
Tissue specificityFound in plasma.
Sequence similaritiesBelongs to the pentaxin family.
Contains 1 pentaxin domain.
- Information by UniProt
- Pentraxin 1, short
- C reactive protein
- C reactive protein pentraxin related
ab157712 has been referenced in 9 publications.
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