Mouse C Reactive Protein ELISA Kit (PTX1) (ab157712)

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Standard Curve

    Key features and details

    • Sensitivity: 0.1691 ng/ml
    • Range: 0.78 ng/ml - 25 ng/ml
    • Sample type: Plasma, Serum, Urine
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Mouse

    Overview

    • Product name

      Mouse C Reactive Protein ELISA Kit (PTX1)
      See all C Reactive Protein kits

    • Detection method

      Colorimetric

    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall < 10%
      Inter-assay
      Sample n Mean SD CV%
      Overall < 10%

    • Sample type

      Urine, Serum, Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      0.1691 ng/ml

    • Range

      0.78 ng/ml - 25 ng/ml

    • Recovery

      Sample specific recovery
      Sample type Average % Range
      Serum > 85 % - %

    • Assay duration

      Multiple steps standard assay

    • Species reactivity

      Reacts with: Mouse

    • Product overview

      Abcam's C Reactive Protein (PTX1) Mouse ELISA kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring C Reactive Protein in mouse biological samples. 

       

      In this assay the C Reactive Protein present in samples reacts with the anti-C Reactive Protein antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-C Reactive Protein antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound C Reactive Protein. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of C Reactive Protein in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of C Reactive Protein in the test sample. The quantity of C Reactive Protein in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

    • Platform

      Microplate

    Properties

    Associated products

      Images

      • Standard Curve
        Standard Curve
        Representative standard curve using ab157712 C Reactive Protein Mouse ELISA kit

      References (9)

      Publishing research using ab157712? Please let us know so that we can cite the reference in this datasheet.

      ab157712 has been referenced in 9 publications.

      • Sayin Z  et al. Evaluation of Boron's Adjuvant Activity in Inactive Bacterin Vaccines Using the Mice Model. Biol Trace Elem Res 199:1037-1043 (2021). PubMed: 32557105
      • Dey S  et al. Sex-specific brain erythropoietin regulation of mouse metabolism and hypothalamic inflammation. JCI Insight 5:N/A (2020). PubMed: 32078583
      • Simanjuntak Y  et al. Preventive effects of folic acid on Zika virus-associated poor pregnancy outcomes in immunocompromised mice. PLoS Pathog 16:e1008521 (2020). PubMed: 32392268
      • Momtazi-Borojeni AA  et al. A simple and rapid-acting approach for the reduction of C-reactive protein. Biomed Pharmacother 109:2305-2308 (2019). PubMed: 30551488
      • Wang D  et al. Caspse-11-GSDMD pathway is required for serum ferritin secretion in sepsis. Clin Immunol N/A:N/A (2019). PubMed: 30731209
      • Suresh S  et al. Erythropoietin modulates bone marrow stromal cell differentiation. Bone Res 7:21 (2019). PubMed: 31666996
      • Pogue AI  et al. Systemic Inflammation in C57BL/6J Mice Receiving Dietary Aluminum Sulfate; Up-Regulation of the Pro-Inflammatory Cytokines IL-6 and TNFa, C-Reactive Protein (CRP) and miRNA-146a in Blood Serum. J Alzheimers Dis Parkinsonism 7:N/A (2017). PubMed: 29354323
      • Schafer S  et al. IL-11 is a crucial determinant of cardiovascular fibrosis. Nature 552:110-115 (2017). ELISA . PubMed: 29160304
      • Symonette CJ  et al. Hyaluronan-phosphatidylethanolamine polymers form pericellular coats on keratinocytes and promote basal keratinocyte proliferation. Biomed Res Int 2014:727459 (2014). PubMed: 25276814

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