Mouse C Reactive Protein ELISA Kit (PTX1) (ab157712)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameMouse C Reactive Protein ELISA Kit (PTX1)
See all C Reactive Protein kits -
Detection methodColorimetric
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Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 10% -
Sample typeUrine, Serum, Plasma
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Assay typeSandwich (quantitative)
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Sensitivity0.169 ng/ml
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Range0.78 ng/ml - 25 ng/ml
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Recovery
Sample specific recovery Sample type Average % Range Serum > 85 % - % -
Assay durationMultiple steps standard assay
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Species reactivityReacts with: Mouse
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Product overview
Abcam's C Reactive Protein (PTX1) Mouse ELISA kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring C Reactive Protein in mouse biological samples.
In this assay the C Reactive Protein present in samples reacts with the anti-C Reactive Protein antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-C Reactive Protein antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound C Reactive Protein. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of C Reactive Protein in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of C Reactive Protein in the test sample. The quantity of C Reactive Protein in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests 100X HRP-conjugated anti-mouse C Reactive Protein antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml Mouse C Reactive Protein Calibrator (Lyophilized) 1 vial Mouse C Reactive Protein ELISA Microplate 1 unit Stop Solution 1 x 12ml -
Research areas
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FunctionDisplays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
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Tissue specificityFound in plasma.
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Sequence similaritiesBelongs to the pentaxin family.
Contains 1 pentaxin domain. -
Cellular localizationSecreted.
- Information by UniProt
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Alternative names
- Pentraxin 1, short
- C reactive protein
- C reactive protein pentraxin related
see all -
Database links
- Entrez Gene: 12944 Mouse
- SwissProt: P14847 Mouse
- Unigene: 28767 Mouse
Applications
Our Abpromise guarantee covers the use of ab157712 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Standard CurveRepresentative standard curve using ab157712 C Reactive Protein Mouse ELISA kit
Protocols
References
This product has been referenced in:
- Pogue AI et al. Systemic Inflammation in C57BL/6J Mice Receiving Dietary Aluminum Sulfate; Up-Regulation of the Pro-Inflammatory Cytokines IL-6 and TNFa, C-Reactive Protein (CRP) and miRNA-146a in Blood Serum. J Alzheimers Dis Parkinsonism 7:N/A (2017). Read more (PubMed: 29354323) »
- Schafer S et al. IL-11 is a crucial determinant of cardiovascular fibrosis. Nature 552:110-115 (2017). ELISA . Read more (PubMed: 29160304) »
