Mouse Catalase Antibody Pair - BSA and Azide free (ab253879)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Mouse
Overview
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Product name
Mouse Catalase Antibody Pair - BSA and Azide free
See all Catalase kits -
Assay type
ELISA set -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Catalase is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Mouse Catalase in sandwich ELISAs and many other pair-based applications.
The pair can be used in variety of assays and platforms including but not limited to:
-Sandwich ELISA
-FRET/TR-FRET/HTRF
-Meso Scale Discovery® (MSD®)
-Bead-based assays
-AlphaLISA®/AlphaScreen®
-DELFIA® immunoassays
-Simoa® and Single Molecule Counting (SMC™) immunoassays
-Multiplex
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
-Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
-Concentration of ~1 mg/ml as measured by the protein A280 method.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for biotin, HRP, gold, and fluorescent dyes.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Please note:
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Antibody properties:
Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration: ~1 mg/ml
Storage buffer: 100% PBS
Form: Liquid
Isotype: IgG
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
For more information see here.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany. -
Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Mouse Catalase Capture Antibody (unconjugated) 1 x 100µg Mouse Catalase Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. -
Involvement in disease
Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions. -
Sequence similarities
Belongs to the catalase family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Peroxisome. - Information by UniProt
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Alternative names
- Cas1
- CAT
- CATA_HUMAN
see all -
Database links
- Entrez Gene: 12359 Mouse
- SwissProt: P24270 Mouse
- Unigene: 4215 Mouse
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab253879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
Images
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Sandwich ELISA - Antibody Pair - BSA and Azide Free (ab253879)To learn more about the advantages of recombinant antibodies see here.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab253879 has not yet been referenced specifically in any publications.
