Overview

  • Product name

    Mouse Cathepsin B ELISA Kit
    See all Cathepsin B kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 5 pg/well
  • Range

    156 pg/well - 10000 pg/well
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Human
  • Product overview

    Abcam’s Mouse Cathepsin B in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Mouse Cathepsin B in cell culture supernatants, serum and plasma (heparin, EDTA).

    A Cathepsin B specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Cathepsin B is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse Cathepsin B amount of sample captured in plate.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab119585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve Using ab119585.

Protocols

References

This product has been referenced in:

  • Mees LM  et al. Low-Intensity Exercise in Mice Is Sufficient to Protect Retinal Function During Light-Induced Retinal Degeneration. Invest Ophthalmol Vis Sci 60:1328-1335 (2019). Read more (PubMed: 30933260) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abreviews
I was interested in detecting secreted cathepsin B in rat hippocampal tissue. Although the kit uses antibodies designed against mouse, mouse and rat cathepsin B share >90% homology. I was able to successfully detect cathepsin B in the supernatant from organotypic hippocampal slices (D.I.V. 10-14) cultured from Wistar rats (P7). The assay was sensitive, showing a positive signal when tested with 200uL of serum-free media, which was washed over the slice for 10 minutes prior to processing. The assay also showed low well-to-well variability when identical samples were run on multiple wells. Serum-free culture media showed little or no reactivity.

Mr. Zahid Padamsey

Verified customer

Submitted Aug 11 2015

Answer

Thank you for contacting Abcam.

I do apologize for the delay in returning this information to you, especially as I image you have already found the results from the mistaken mixing ofthe biotinylation solution with sample buffer instead of antibody dilution buffer. We have no data regarding this and as such we would not recommend proceeding as your may have high background. However if you have found this to be different please let us know.

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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