Key features and details
- Sensitivity: 5 pg/well
- Range: 156 pg/well - 10000 pg/well
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse Cathepsin B ELISA Kit
See all Cathepsin B kits
Intra-assay Sample n Mean SD CV% 1 16 242pg/ml 14.76 6.1% 2 16 1828pg/ml 100.54 5.5% 3 16 4749pg/ml 370.42 7.8% Inter-assay Sample n Mean SD CV% 1 24 229pg/ml 13.96 6.1% 2 24 1916pg/ml 128.37 6.7% 3 24 4534pg/ml 439.79 9.7%
Sample typeCell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma
Assay typeSandwich (quantitative)
Sensitivity< 5 pg/well
Range156 pg/well - 10000 pg/well
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Does not react with: Human
Abcam’s Mouse Cathepsin B in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Mouse Cathepsin B in cell culture supernatants, serum and plasma (heparin, EDTA).
A Cathepsin B specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Cathepsin B is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse Cathepsin B amount of sample captured in plate.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-mouse Cathepsin B antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti-mouse Cathepsin B antibody 1 x 130µl Lyophilized recombinant mouse Cathepsin B standard 2 x 10ng Plate Seal 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml
FunctionThiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
Sequence similaritiesBelongs to the peptidase C1 family.
Cellular localizationLysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Amyloid precursor protein secretase
- APP secretase
ab119585 has been referenced in 1 publication.
- Mees LM et al. Low-Intensity Exercise in Mice Is Sufficient to Protect Retinal Function During Light-Induced Retinal Degeneration. Invest Ophthalmol Vis Sci 60:1328-1335 (2019). PubMed: 30933260