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Phoned because they accidentally mixed the biotinylation solution with sample buffer instead of antibody dilution buffer (see page 12 of protocol booklet). Would like to know if it is ok to proceed or what effects this might have?
Asked on Mar 09 2012
Thank you for contacting Abcam.
I do apologize for the delay in returning this information to you, especially as I image you have already found the results from the mistaken mixing ofthe biotinylation solution with sample buffer instead of antibody dilution buffer. We have no data regarding this and as such we would not recommend proceeding as your may have high background. However if you have found this to be different please let us know.
I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.
Answered on Mar 09 2012