Mouse Cathepsin D ELISA Kit (ab213845)
- Datasheet
- References
- Protocols
Overview
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Product name
Mouse Cathepsin D ELISA Kit
See all Cathepsin D kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Sample 1 16 0.45ng/ml 0.023 5.1% Sample 2 16 1.81ng/ml 0.109 6% Sample 3 16 8.8ng/ml 0.545 6.2% Inter-assay Sample n Mean SD CV% Sample 1 24 0.47ng/ml 0.03 6.3% Sample 2 24 2.13ng/ml 0.126 5.9% Sample 3 24 7.99ng/ml 0.336 4.2% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Homogenate -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
312 pg/ml - 20000 pg/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
The Mouse Cathepsin D Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213845) is designed for the quantitative measurement of Mouse Cathepsin D in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Cathepsin D has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: I21-L410) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Cathepsin D amount of sample captured in plate.
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Notes
Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Mouse Cathepsin D coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti- Mouse Cathepsin D antibody 1 x 130µl Lyophilized recombinant Mouse Cathepsin D standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml -
Research areas
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Function
Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. -
Tissue specificity
Expressed in the aorta extrcellular space (at protein level). -
Involvement in disease
Ceroid lipofuscinosis, neuronal, 10 -
Sequence similarities
Belongs to the peptidase A1 family.
Contains 1 peptidase A1 domain. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380). - Information by UniProt
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Alternative names
- CatD
- CATD_HUMAN
- Cathepsin D
see all -
Database links
- Entrez Gene: 13033 Mouse
- SwissProt: P18242 Mouse
- Unigene: 231395 Mouse
Applications
Our Abpromise guarantee covers the use of ab213845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Mouse Cathepsin D ELISA Kit (ab213845) Standard Curve.Mouse Cathepsin D ELISA Kit (ab213845) Standard Curve.
Protocols
References
ab213845 has not yet been referenced specifically in any publications.
