Mouse Cathepsin D ELISA Kit (ab213845)

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Key features and details

  • Sensitivity: 10 pg/ml
  • Range: 312 pg/ml - 20000 pg/ml
  • Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse Cathepsin D ELISA Kit
    See all Cathepsin D kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 844pg/ml 63.3 = 7.5%
    2 16 1778pg/ml 99.56 = 5.6%
    3 16 7768pg/ml 458.31 = 5.9%
    Inter-assay
    Sample n Mean SD CV%
    1 24 898pg/ml 77.43 = 8.5%
    2 24 1799pg/ml 133.12 = 7.4%
    3 24 8034pg/ml 514.17 = 6.4%

  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    < 10 pg/ml

  • Range

    312 pg/ml - 20000 pg/ml

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    The Mouse Cathepsin D Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213845) is designed for the quantitative measurement of Mouse Cathepsin D in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). 


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for Cathepsin D has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: I21-L410) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Cathepsin D amount of sample captured in plate.

  • Notes

    Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Mouse Cathepsin D coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Mouse Cathepsin D antibody 1 x 130µl
    Lyophilized recombinant Mouse Cathepsin D standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml

  • Research areas

  • Function

    Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.

  • Tissue specificity

    Expressed in the aorta extrcellular space (at protein level).

  • Involvement in disease

    Ceroid lipofuscinosis, neuronal, 10

  • Sequence similarities

    Belongs to the peptidase A1 family.
    Contains 1 peptidase A1 domain.

  • Post-translational
    modifications

    N- and O-glycosylated.

  • Cellular localization

    Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
  • Information by UniProt

  • Alternative names

    • CatD
    • CATD_HUMAN
    • Cathepsin D
    • Cathepsin D heavy chain
    • CD
    • Ceroid lipofuscinosis neuronal 10
    • CLN10
    • CPSD
    • ctsd
    • Epididymis secretory sperm binding protein Li 130P
    • HEL S 130P
    • Lysosomal aspartyl peptidase
    • Lysosomal aspartyl protease
    • MGC2311
    see all

  • Database links

    Associated products

      Images

      • Mouse Cathepsin D ELISA Kit (ab213845) Standard Curve.
        Mouse Cathepsin D ELISA Kit (ab213845) Standard Curve.

        Mouse Cathepsin D ELISA Kit (ab213845) Standard Curve.

      References (0)

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      ab213845 has not yet been referenced specifically in any publications.

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