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    mouse-cathepsin-d-elisa-kit-ab213845.pdf

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Neuroscience Cell Adhesion Proteins Membrane Proteins
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Mouse Cathepsin D ELISA Kit (ab213845)

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  • SDS
  • Protocol Booklet
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Mouse Cathepsin D ELISA Kit (ab213845) Standard Curve.

    Key features and details

    • Sensitivity: 10 pg/ml
    • Range: 312 pg/ml - 20000 pg/ml
    • Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Mouse

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    Overview

    • Product name

      Mouse Cathepsin D ELISA Kit
      See all Cathepsin D kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      1 16 844pg/ml 63.3 = 7.5%
      2 16 1778pg/ml 99.56 = 5.6%
      3 16 7768pg/ml 458.31 = 5.9%
      Inter-assay
      Sample n Mean SD CV%
      1 24 898pg/ml 77.43 = 8.5%
      2 24 1799pg/ml 133.12 = 7.4%
      3 24 8034pg/ml 514.17 = 6.4%
    • Sample type

      Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      < 10 pg/ml
    • Range

      312 pg/ml - 20000 pg/ml
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Mouse
    • Product overview

      The Mouse Cathepsin D Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213845) is designed for the quantitative measurement of Mouse Cathepsin D in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). 


      The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for Cathepsin D has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: I21-L410) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Cathepsin D amount of sample captured in plate.

    • Notes

      Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.

    • Platform

      Pre-coated microplate (12 x 8 well strips)

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components Identifier 1 x 96 tests
      ABC Diluent Buffer Blue Cap 1 x 12ml
      Adhesive Plate Seal 4 units
      Antibody Diluent Buffer Green Cap 1 x 12ml
      Anti-Mouse Cathepsin D coated Microplate (12 x 8 wells) 1 unit
      Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl
      Biotinylated anti- Mouse Cathepsin D antibody 1 x 100µl
      Lyophilized recombinant Mouse Cathepsin D standard 2 vials
      Sample Diluent Buffer Green Cap 1 x 30ml
      TMB Color Developing Agent Black Cap 1 x 10ml
      TMB Stop Solution Yellow Cap 1 x 10ml
    • Research areas

      • Neuroscience
      • Cell Adhesion Proteins
      • Membrane Proteins
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • ECM Enzymes
      • Other Enzymes
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Extracellular matrix
      • Other
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteolytic enzymes
      • Cysteine protease
      • Cathepsins
      • Neuroscience
      • Diseases
      • Neuroscience
      • Processes
    • Function

      Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
    • Tissue specificity

      Expressed in the aorta extrcellular space (at protein level).
    • Involvement in disease

      Ceroid lipofuscinosis, neuronal, 10
    • Sequence similarities

      Belongs to the peptidase A1 family.
      Contains 1 peptidase A1 domain.
    • Post-translational
      modifications

      N- and O-glycosylated.
    • Cellular localization

      Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
    • Target information above from: UniProt accession P07339 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • CatD
      • CATD_HUMAN
      • Cathepsin D
      • Cathepsin D heavy chain
      • CD
      • Ceroid lipofuscinosis neuronal 10
      • CLN10
      • CPSD
      • ctsd
      • Epididymis secretory sperm binding protein Li 130P
      • HEL S 130P
      • Lysosomal aspartyl peptidase
      • Lysosomal aspartyl protease
      • MGC2311
      see all
    • Database links

      • Entrez Gene: 13033 Mouse
      • SwissProt: P18242 Mouse

      Images

      • Mouse Cathepsin D ELISA Kit (ab213845) Standard Curve.
        Mouse Cathepsin D ELISA Kit (ab213845) Standard Curve.

        Mouse Cathepsin D ELISA Kit (ab213845) Standard Curve.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab213845? Please let us know so that we can cite the reference in this datasheet.

      ab213845 has been referenced in 1 publication.

      • Martinelli S  et al. Stress-primed secretory autophagy promotes extracellular BDNF maturation by enhancing MMP9 secretion. Nat Commun 12:4643 (2021). PubMed: 34330919

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