Mouse Cathepsin D ELISA Kit (ab239420)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 775 pg/ml
- Range: 4.22 ng/ml - 270 ng/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Cathepsin D ELISA Kit
See all Cathepsin D kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% serum 8 4.1% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
775 pg/ml -
Range
4.22 ng/ml - 270 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 109 % - % Hep Plasma 94 % - % EDTA Plasma 104 % - % Cit plasma 109 % - % -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Cathepsin D ELISA Kit (ab239420) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cathepsin D protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Cathepsin D with 775 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Cathepsin D Capture Antibody 1 x 600µl 10X Mouse Cathepsin D Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse Cathepsin D Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. -
Tissue specificity
Expressed in the aorta extrcellular space (at protein level). -
Involvement in disease
Ceroid lipofuscinosis, neuronal, 10 -
Sequence similarities
Belongs to the peptidase A1 family.
Contains 1 peptidase A1 domain. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380). - Information by UniProt
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Alternative names
- CatD
- CATD_HUMAN
- Cathepsin D
see all -
Database links
- Entrez Gene: 13033 Mouse
- SwissProt: P18242 Mouse
Associated products
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Alternative Versions
Images
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Other - Mouse Cathepsin D ELISA Kit (ab239420)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Cathepsin D standard curve in Sample Diluent NS.The Cathepsin D standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native Cathepsin D in mouse serum and plasma samples.The concentrations of Cathepsin D were measured in duplicate, interpolated from the Cathepsin D standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and plasma (Heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cathepsin D concentration was determined to be 366.2 ng/mL in serum, 300.8 ng/mL in plasma (citrate), 213.9 ng/mL in plasma (EDTA), and 446.9 ng/mL in plasma (Heparin).
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Sandwich ELISA - Mouse Cathepsin D ELISA Kit (ab239420)To learn more about the advantages of recombinant antibodies see here.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab239420 has been referenced in 2 publications.
- Zhu RR et al. Inhibition of the Notch1 pathway induces peripartum cardiomyopathy. J Cell Mol Med 24:7907-7914 (2020). PubMed: 32529705
- Al-Salam S et al. Galectin-3: A Cardiomyocyte Antiapoptotic Mediator at 24-Hour Post Myocardial Infarction. Cell Physiol Biochem 54:287-302 (2020). PubMed: 32246616