Mouse Cathepsin D ELISA Kit (ab239420)
- Datasheet
- References
- Protocols
Overview
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Product name
Mouse Cathepsin D ELISA Kit
See all Cathepsin D kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% serum 8 4.1% -
Sample type
Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
775 pg/ml -
Range
4.22 ng/ml - 270 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 109 % - % Heparin Plasma 94 % - % EDTA Plasma 104 % - % Citrate Plasma 109 % - % -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Cathepsin D in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Cathepsin D protein in mouse serum and plasma samples.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Cathepsin D is a lysosomal aspartyl protease that is activated at low pH. Mutations in Cathepsin D have been linked to pathogenesis of diseases including breast cancer and Alzheimer disease.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml 10X Mouse Cathepsin D Capture Antibody 1 x 600µl 10X Mouse Cathepsin D Detector Antibody 1 x 600µl Mouse Cathepsin D Lyophilized Recombinant Protein 1 x 600 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. -
Tissue specificity
Expressed in the aorta extrcellular space (at protein level). -
Involvement in disease
Ceroid lipofuscinosis, neuronal, 10 -
Sequence similarities
Belongs to the peptidase A1 family.
Contains 1 peptidase A1 domain. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380). - Information by UniProt
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Alternative names
- CatD
- CATD_HUMAN
- Cathepsin D
see all -
Database links
- Entrez Gene: 13033 Mouse
- SwissProt: P18242 Mouse
- Unigene: 231395 Mouse
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab239420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Other - Mouse Cathepsin D ELISA Kit (ab239420)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Cathepsin D standard curve in Sample Diluent NS.The Cathepsin D standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native Cathepsin D in mouse serum and plasma samples.The concentrations of Cathepsin D were measured in duplicate, interpolated from the Cathepsin D standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and plasma (Heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cathepsin D concentration was determined to be 366.2 ng/mL in serum, 300.8 ng/mL in plasma (citrate), 213.9 ng/mL in plasma (EDTA), and 446.9 ng/mL in plasma (Heparin).
Protocols
References
ab239420 has not yet been referenced specifically in any publications.
