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    mouse-cathepsin-d-elisa-kit-ab239420.pdf

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RecombinantSimpleStep

Mouse Cathepsin D ELISA Kit (ab239420)

  • Datasheet
  • SDS
  • Protocol Booklet
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Other - Mouse Cathepsin D ELISA Kit (ab239420)
  • Example of mouse Cathepsin D standard curve in Sample Diluent NS.
  • Interpolated concentrations of native Cathepsin D in mouse serum and plasma samples.
  • Sandwich ELISA - Mouse Cathepsin D ELISA Kit (ab239420)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 775 pg/ml
  • Range: 4.22 ng/ml - 270 ng/ml
  • Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

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Overview

  • Product name

    Mouse Cathepsin D ELISA Kit
    See all Cathepsin D kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    serum 8 4.1%
  • Sample type

    Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    775 pg/ml
  • Range

    4.22 ng/ml - 270 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 109 % - %
    Hep Plasma 94 % - %
    EDTA Plasma 104 % - %
    Cit plasma 109 % - %
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Mouse Cathepsin D ELISA Kit (ab239420) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cathepsin D protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Cathepsin D with 775 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse Cathepsin D Capture Antibody 1 x 600µl
    10X Mouse Cathepsin D Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BR 1 x 6ml
    Mouse Cathepsin D Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Membrane Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • Other Enzymes
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • Other
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Cysteine protease
    • Cathepsins
    • Neuroscience
    • Diseases
    • Neuroscience
    • Processes
  • Function

    Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
  • Tissue specificity

    Expressed in the aorta extrcellular space (at protein level).
  • Involvement in disease

    Ceroid lipofuscinosis, neuronal, 10
  • Sequence similarities

    Belongs to the peptidase A1 family.
    Contains 1 peptidase A1 domain.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
  • Target information above from: UniProt accession P07339 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • CatD
    • CATD_HUMAN
    • Cathepsin D
    • Cathepsin D heavy chain
    • CD
    • Ceroid lipofuscinosis neuronal 10
    • CLN10
    • CPSD
    • ctsd
    • Epididymis secretory sperm binding protein Li 130P
    • HEL S 130P
    • Lysosomal aspartyl peptidase
    • Lysosomal aspartyl protease
    • MGC2311
    see all
  • Database links

    • Entrez Gene: 13033 Mouse
    • SwissProt: P18242 Mouse

    Associated products

    • Alternative Versions

      • Mouse Cathepsin D Antibody Pair - BSA and Azide free (ab244163)

    Images

    • Other - Mouse Cathepsin D ELISA Kit (ab239420)
      Other - Mouse Cathepsin D ELISA Kit (ab239420)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of mouse Cathepsin D standard curve in Sample Diluent NS.
      Example of mouse Cathepsin D standard curve in Sample Diluent NS.
      The Cathepsin D standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
    • Interpolated concentrations of native Cathepsin D in mouse serum and plasma samples.
      Interpolated concentrations of native Cathepsin D in mouse serum and plasma samples.

      The concentrations of Cathepsin D were measured in duplicate, interpolated from the Cathepsin D standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and plasma (Heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cathepsin D concentration was determined to be 366.2 ng/mL in serum, 300.8 ng/mL in plasma (citrate), 213.9 ng/mL in plasma (EDTA), and 446.9 ng/mL in plasma (Heparin).

    • Sandwich ELISA - Mouse Cathepsin D ELISA Kit (ab239420)
      Sandwich ELISA - Mouse Cathepsin D ELISA Kit (ab239420)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab239420? Please let us know so that we can cite the reference in this datasheet.

    ab239420 has been referenced in 2 publications.

    • Zhu RR  et al. Inhibition of the Notch1 pathway induces peripartum cardiomyopathy. J Cell Mol Med 24:7907-7914 (2020). PubMed: 32529705
    • Al-Salam S  et al. Galectin-3: A Cardiomyocyte Antiapoptotic Mediator at 24-Hour Post Myocardial Infarction. Cell Physiol Biochem 54:287-302 (2020). PubMed: 32246616

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