Overview

  • Product name

    Mouse Cathepsin D ELISA Kit
    See all Cathepsin D kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    serum 8 4.1%
  • Sample type

    Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    775 pg/ml
  • Range

    4.22 ng/ml - 270 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 109 % - %
    Heparin Plasma 94 % - %
    EDTA Plasma 104 % - %
    Citrate Plasma 109 % - %

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Cathepsin D in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Cathepsin D protein in mouse serum and plasma samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Cathepsin D is a lysosomal aspartyl protease that is activated at low pH. Mutations in Cathepsin D have been linked to pathogenesis of diseases including breast cancer and Alzheimer disease.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab239420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • The Cathepsin D standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
  • The concentrations of Cathepsin D were measured in duplicate, interpolated from the Cathepsin D standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and plasma (Heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cathepsin D concentration was determined to be 366.2 ng/mL in serum, 300.8 ng/mL in plasma (citrate), 213.9 ng/mL in plasma (EDTA), and 446.9 ng/mL in plasma (Heparin).

Protocols

References

ab239420 has not yet been referenced specifically in any publications.

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