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    mouse-ccl28-elisa-kit-ab210578.pdf

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Immunology Innate Immunity Chemokines Beta Chemokines (CC)
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RecombinantSimpleStep

Mouse CCL28 ELISA Kit (ab210578)

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  • SDS
  • Protocol Booklet
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Other - Mouse CCL28 ELISA Kit (ab210578)
  • Example of mouse CCL28 standard curve in Sample Diluent NS.
  • Example of mouse CCL28 standard curve in Sample Diluent 20BP.
  • Example of mouse CCL28 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.
  • Interpolated concentrations of spiked CCL28 in mouse serum and plasma samples.
  • Interpolated concentrations of native CCL28 in mouse serum and plasma samples.
  • Sandwich ELISA - Mouse CCL28 ELISA Kit (ab210578)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 4.7 pg/ml
  • Range: 62.5 pg/ml - 4000 pg/ml
  • Sample type: Cell culture extracts, Cell culture supernatant, Plasma, Serum, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

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Protein
Recombinant Mouse CCL28/MEC protein (ab50171)

View more associated products

Overview

  • Product name

    Mouse CCL28 ELISA Kit
    See all CCL28/MEC kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Saliva 8 2.6%
    Inter-assay
    Sample n Mean SD CV%
    Saliva 3 6.9%
  • Sample type

    Cell culture supernatant, Serum, Plasma, Cell culture extracts, Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    4.7 pg/ml
  • Range

    62.5 pg/ml - 4000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 116 115% - 118%
    Serum 111 99% - 118%
    Hep Plasma 99 96% - 100%
    EDTA Plasma 109 105% - 112%
    Cit plasma 99 94% - 105%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Rat, Human
  • Product overview

    Mouse CCL28 ELISA Kit (ab210578) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL28 protein in cell culture extracts, cell culture supernatant, plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CCL28 with 4.7 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Mouse CCL28 is a member of the CC subfamily of chemokines. Mucosal epithelial cells such as exocrine glands, trachea, and colon express mouse CCL28 protein. In lower levels, mouse CCL28 is also found in brain, spleen and lung tissues. CCL28 chemoattracts resting CD4 and CD8 cells, as well as IgA antibody secreting cells. Furthermore, CCL28 plays a role in extravasation of IgA antibody secreting cells in the small intestine, colon, and lactating mammary gland. Human and mouse CCL28 share an overall 66% amino acid sequence identity, with an 83% amino acid sequence identity in their mature regions.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse CCL28 Capture Antibody 1 x 600µl
    10X Mouse CCL28 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 5BI 1 x 6ml
    Mouse CCL28 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NBP 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Beta Chemokines (CC)
    • Immunology
    • Innate Immunity
    • Cytokines
    • Other
    • Metabolism
    • Types of disease
    • Obesity
  • Function

    Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner.
  • Tissue specificity

    Preferentially expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. Also found in prostate, spleen, thyroid, psoriasis skin and in lower levels in peripheral blood leukocytes, small intestine, Peyer patches, stomach and normal skin.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q9NRJ3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • C C motif chemokine ligand 28
    • C-C motif chemokine 28
    • CC chemokine CCL28
    • CCK 1
    • CCK1
    • CCK1 protein
    • CCL 28
    • CCL28
    • CCL28_HUMAN
    • Chemokine (C-C motif) ligand 28
    • chemokine (C-C motif) ligand 28 splice variant chi
    • MEC
    • Mucosae associated epithelial chemokine
    • Mucosae-associated epithelial chemokine
    • Protein CCK1
    • SCYA28
    • Small inducible cytokine A28 [Precursor]
    • small inducible cytokine subfamily A (Cys-Cys), member 28
    • Small-inducible cytokine A28
    see all
  • Database links

    • Entrez Gene: 56838 Mouse
    • SwissProt: Q9JIL2 Mouse
    • Unigene: 143745 Mouse

    Associated products

    • ELISA kits

      • Mouse CCL28 Matched Antibody Pair Kit (ab216061)

    Images

    • Other - Mouse CCL28 ELISA Kit (ab210578)
      Other - Mouse CCL28 ELISA Kit (ab210578)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of mouse CCL28 standard curve in Sample Diluent NS.
      Example of mouse CCL28 standard curve in Sample Diluent NS.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Example of mouse CCL28 standard curve in Sample Diluent 20BP.
      Example of mouse CCL28 standard curve in Sample Diluent 20BP.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Example of mouse CCL28 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.
      Example of mouse CCL28 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of spiked CCL28 in mouse serum and plasma samples.
      Interpolated concentrations of spiked CCL28 in mouse serum and plasma samples.

      The concentrations of CCL28 were measured in duplicates, interpolated from the CCL28 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL28 concentration was determined to be 4,718.7 pg/mL in serum, 4,395.5 pg/mL in plasma (citrate), 4,697.8 pg/mL in plasma (EDTA) and 5,130.8 pg/mL in plasma (heparin).

    • Interpolated concentrations of native CCL28 in mouse serum and plasma samples.
      Interpolated concentrations of native CCL28 in mouse serum and plasma samples.

      The concentrations of CCL28 were measured in duplicates, interpolated from the CCL28 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL28 concentration was determined to be 461.6 pg/mL in serum, 615.2 pg/mL in plasma (citrate), 609.5 pg/mL in plasma (EDTA), and 390.0 pg/mL in plasma (heparin).

    • Sandwich ELISA - Mouse CCL28 ELISA Kit (ab210578)
      Sandwich ELISA - Mouse CCL28 ELISA Kit (ab210578)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab210578? Please let us know so that we can cite the reference in this datasheet.

    ab210578 has not yet been referenced specifically in any publications.

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