Mouse CCL28 ELISA Kit (ab210578)
- Datasheet
- References
- Protocols
Overview
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Product name
Mouse CCL28 ELISA Kit
See all CCL28/MEC kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Saliva 8 2.6% Inter-assay Sample n Mean SD CV% Saliva 3 6.9% -
Sample type
Cell culture supernatant, Serum, Plasma, Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
4.7 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 116 115% - 118% Serum 111 99% - 118% Hep Plasma 99 96% - 100% EDTA Plasma 109 105% - 112% Cit plasma 99 94% - 105% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Rat, Human -
Product overview
CCL28 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of mouse CCL28 protein in serum, plasma, cell culture supernatants, and cell and tissue extracts only.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Sensitivity:
Sample Diluent NS MDD = 4.7 pg/mL.
1X Cell Extraction Buffer PTR MDD = 4.8 pg/mL.
Sample Diluent 20 BP MDD = 17.6 pg/mL.
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Notes
Mouse CCL28 is a member of the CC subfamily of chemokines. Mucosal epithelial cells such as exocrine glands, trachea, and colon express mouse CCL28 protein. In lower levels, mouse CCL28 is also found in brain, spleen and lung tissues. CCL28 chemoattracts resting CD4 and CD8 cells, as well as IgA antibody secreting cells. Furthermore, CCL28 plays a role in extravasation of IgA antibody secreting cells in the small intestine, colon, and lactating mammary gland. Human and mouse CCL28 share an overall 66% amino acid sequence identity, with an 83% amino acid sequence identity in their mature regions.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse CCL28 Capture Antibody 1 x 600µl 10X Mouse CCL28 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Mouse CCL28 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NBP 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner. -
Tissue specificity
Preferentially expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. Also found in prostate, spleen, thyroid, psoriasis skin and in lower levels in peripheral blood leukocytes, small intestine, Peyer patches, stomach and normal skin. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C C motif chemokine ligand 28
- C-C motif chemokine 28
- CC chemokine CCL28
see all -
Database links
- Entrez Gene: 56838 Mouse
- SwissProt: Q9JIL2 Mouse
- Unigene: 143745 Mouse
Associated products
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ELISA kits
Applications
Our Abpromise guarantee covers the use of ab210578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Other - Mouse CCL28 ELISA Kit (ab210578)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse CCL28 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse CCL28 standard curve in Sample Diluent 20BP.Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse CCL28 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked CCL28 in mouse serum and plasma samples.The concentrations of CCL28 were measured in duplicates, interpolated from the CCL28 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL28 concentration was determined to be 4,718.7 pg/mL in serum, 4,395.5 pg/mL in plasma (citrate), 4,697.8 pg/mL in plasma (EDTA) and 5,130.8 pg/mL in plasma (heparin).
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Interpolated concentrations of native CCL28 in mouse serum and plasma samples.The concentrations of CCL28 were measured in duplicates, interpolated from the CCL28 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL28 concentration was determined to be 461.6 pg/mL in serum, 615.2 pg/mL in plasma (citrate), 609.5 pg/mL in plasma (EDTA), and 390.0 pg/mL in plasma (heparin).
Protocols
References
ab210578 has not yet been referenced specifically in any publications.
