Overview

  • Product name
    Mouse CD137 ELISA Kit
    See all CD137 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    supernatant 5 3.9%
    Inter-assay
    Sample n Mean SD CV%
    supernatant 3 4.8%
  • Sample type
    Cell culture supernatant, Serum, Plasma, Cell culture extracts, Tissue Extracts
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    14 pg/ml
  • Range
    78.125 pg/ml - 5000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 108.8 99.9% - 114.3%
    Cell culture media 94.5 88.4% - 103.7%
    EDTA Plasma 106.3 103.5% - 110.1%
    Citrate Plasma 120.5 116.6% - 124.1%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Rat, Rabbit, Goat, Guinea pig, Hamster, Cow, Dog, Human, Pig
  • Product overview

    Abcam’s CD137 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD137 protein in mouse serum, plasma cell culture supernatant, cell and tissue extract samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:


    Samples diluted in Sample Dilunet NS: 14 pg/mL.


    Samples diluted in 1X Cell Extraction Buffer PTR: 48 pg/mL.

  • Notes

    CD137 (also known as 4-1BB and Tumor necrosis factor receptor superfamily member 9 (TNRSF9)), a member of tumor necrosis factor receptor family, is a plasma membrane receptor for TNFSF9/4-1BBL. CD137 is expressed on the surface of activated T lymphocytes. CD137 is principally a homodimer, but also found as a monomer. CD137 generates either co-stimulatory signals leading to T cell activation and proliferation, or death signals, and may promote tumor rejection in vivo. Soluble forms of CD137 exist, these are generated by proteolytic cleavage or alternative splicing. In the years, functional expression of CD137 was noted on several non-T cells including monocytes, neutrophils, macrophages, B cells and natural killer (NK) cells.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse CD137 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Mouse CD137 Lyophilized Recombinant Protein 2 vials
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 5BR 1 x 6ml
    Mouse CD137 Capture Antibody (lyophilized) 1 vial
    Plate Seals 1 unit
    Sample Diluent NS 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas
  • Function
    Receptor for TNFSF14/4-1BBL. Possibly active during T cell activation.
  • Tissue specificity
    Expressed on the surface of activated T-cells.
  • Sequence similarities
    Contains 4 TNFR-Cys repeats.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Alternative names
    • 4-1BB
    • 4-1BB ligand receptor
    • CD137
    • CD137 antigen
    • CDw137
    • Homolog of mouse 4 1BB
    • ILA
    • Induced by lymphocyte activation (ILA)
    • Interleukin activated receptor homolog of mouse Ly63
    • Receptor protein 4 1BB
    • T-cell antigen 4-1BB homolog
    • T-cell antigen ILA
    • TNFRSF9
    • TNR9_HUMAN
    • Tumor necrosis factor receptor superfamily member 9
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab205577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • ELISA Protocol Summary
  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of CD137 were measured in duplicates, interpolated from the CD137  standard curves and corrected for sample dilution. Note that 1X Diluted serum and plasma samples were pre-diluted to 20%. Note that 1X Diluted Raw 264.7 day 3 cell culture supernatant samples were pre-diluted to 10%. The interpolated, dilution factor-corrected values are plotted in pg of CD137  per mL of neat sample (mean +/- SD, n=2).

  • EL4.IL2 cells were serum starved for 24 hours and then cultured in the absence or presence of 1.5% PHA and 10 ng/mL PMA for 48 hours. Mouse spleen cells were cultured in the presence of 5 µg/mL LPS for 6 days. The cell culture supernatants were prepared. The concentrations of CD137 were measured in the diluted cell culture supernatant samples in duplicates, interpolated from the CD137 standard curve and corrected for sample dilution. Note that EL4.IL2 cell culture supernatant samples were analyzed neat, 2X and 4X diluted. Note that mouse spleen cell culture supernatant samples were analyzed 4X, 8X and 16X diluted. The interpolated, dilution factor-corrected values are plotted in pg of CD137 per mL of neat supernatant (mean +/- SD, n=3).

  • EL4.IL2 cells were cultured in the absence of serum for 3 days. NIH3T3, L929, D10G4.1 and Raw 264.7 cells were cultured in the presence of serum for 3 days. The cell culture supernatants were prepared. The concentrations of CD137 were measured in the diluted cell culture supernatant samples in duplicates, interpolated from the 4-1BB standard curve and corrected for sample dilution. Note that EL4.IL2 cell culture supernatant samples were analyzed neat and 2X diluted. Note that NIH/3T3 cell culture supernatant samples were analyzed neat. Note that L929 cell culture supernatant samples were analyzed neat, 2X and 4X diluted. Note that D10G4.1 cell culture supernatant samples were analyzed 2X, 4X and 8X diluted. Note that Raw 264.7 cell culture supernatant samples were analyzed 10X, 20X and 40X diluted. The interpolated, dilution factor-corrected values are plotted in pg of CD137 per mL of neat supernatant (mean +/- SD, n=1-3).

Protocols

References

ab205577 has not yet been referenced specifically in any publications.

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