Overview

  • Product name

    Mouse CD23 ELISA Kit
    See all CD23 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 7.9%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 9.5%
  • Sample type

    Cell culture supernatant, Serum, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    2 pg/ml
  • Range

    31.25 pg/ml - 2000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 126.1 119.2% - 133.4%
    Cell culture media 120.7 117% - 123.3%
    EDTA Plasma 89.2 85.8% - 92.3%
    Citrate Plasma 81.3 77.8% - 83.9%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Rat, Rabbit, Hamster, Dog, Human, Pig
  • Product overview

    Abcam’s CD23 (Fc epsilon RII) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD23 protein in mouse serum, plasma and cell culture supernatant samples.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

     

    Sensitivity: Samples diluted in Sample Diluent NS: 2 pg/mL

                         Samples diluted in Sample Diluent 25BS: 27 pg/mL

  • Notes

    CD23 is a low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. It has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen). It is a 47 kDa type II transmembrane glycoprotein containing a short N-terminal cytoplasmic tail, a transmembrane domain, and extracellular domain containing C-type lectin domain. Two membrane isoforms and soluble CD23 fragements exist.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab206984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of CD23 were measured in duplicates, interpolated from the CD23 standard curves and corrected for sample dilution. Note that 1X Diluted serum samples were pre-diluted to 2.5%. Note that 1X Diluted plasma samples were pre-diluted to 5%. Note that 1X Diluted Mouse spleen Day 5 cell culture supernatant samples were pre-diluted to 25%. The interpolated, dilution factor-corrected values are graphed in pg of CD23 per mL of neat sample (mean +/- SD, n=2).

Protocols

References

ab206984 has not yet been referenced specifically in any publications.

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