Overview

  • Product name

    Mouse CD27 ELISA Kit
    See all CD27 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 5.3%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 2%
  • Sample type

    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    10.9 pg/ml
  • Range

    31.3 pg/ml - 2000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 104 98% - 108%
    Serum 100 93% - 106%
    Heparin Plasma 105 99% - 109%
    EDTA Plasma 90 79% - 99%
    Citrate Plasma 94 88% - 98%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Rat, Cow, Human
  • Product overview

    CD27 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD27 protein in mouse serum, plasma, and cell culture supernatant samples


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    CD27 is a member of the TNF-receptor superfamily involved in T cell long term immunity. It is also involved in the regulation of B cell activation and immunoglobulin synthesis through the NF-κB and MAPK8/JNK pathways. CD27 may be involved in apoptosis due to interactions with SIVA, a proapoptotic protein.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab233619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of CD27 were measured in duplicates, interpolated from the CD27 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (citrate) 12.5%, plasma (heparin) 12.5%, and plasma (EDTA) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD27 concentration was determined to be 14.3 ng/mL in serum, 11.9 ng/mL in plasma (citrate), 13.4 g/ml in plasma (heparin), and 10.8 ng/ml in plasma (EDTA).

  • The concentrations of CD27 were measured in duplicates, interpolated from the CD27 standard curves and corrected for sample dilution. Undiluted samples are as follows: untreated EL4-IL2 supernatant 100% and treated EL4-IL2 supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD27 concentration was determined to be 426 pg/mL in untreated EL4-IL2 supernatant and 1,978 pg/ml in treated EL4-IL2 supernatant.

Protocols

References

ab233619 has not yet been referenced specifically in any publications.

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