Key features and details
- Sensitivity: 10 pg/ml
- Range: 12.29 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse CD30 ELISA Kit
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 10 pg/ml
Range12.29 pg/ml - 3000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 77.29 69% - 87% Serum 75.99 68% - 84% Plasma 74.88 67% - 83%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Abcam’s CD30 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CD30 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse CD30 coated on a 96- well plate. Standards and samples are pipetted into the wells and CD30 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse CD30 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD30 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse CD30 (lyophilized) 2 vials CD30 Microplate (12 strips x 8 wells) 1 unit Recombinant Mouse CD30 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
- SwissProt: Q60846 Mouse
ab113341 has not yet been referenced specifically in any publications.