Key features and details
- Sensitivity: 30 pg/ml
- Range: 27.43 pg/ml - 20000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse CD36 ELISA Kit
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 30 pg/ml
Range27.43 pg/ml - 20000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 78.9 69% - 87% Serum 90.93 80% - 101% Plasma 80.53 71% - 87%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Abcam’s CD36 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD36 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse CD36 coated on a 96- well plate. Standards and samples are pipetted into the wells and CD36 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse CD36 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD36 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse CD36 2 vials CD36 Microplate (12 strips x 8 wells) 1 unit Recombinant Mouse CD36 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
RelevanceCD36 is a cell surface class B scavenger receptor. CD36 is preferentially found within lipid rafts, which facilitates its association with receptors, signalling and adapter molecules. CD36 binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. May function as a cell adhesion molecule. Directly mediates cyto adherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport.
- CD36 antigen
- CD36 antigen (collagen type I receptor thrombospondin receptor)
- CD36 molecule
- SwissProt: Q08857 Mouse
ab100673 has been referenced in 1 publication.
- Moya-Pérez A et al. Hepatic molecular responses to Bifidobacterium pseudocatenulatum CECT 7765 in a mouse model of diet-induced obesity. Nutr Metab Cardiovasc Dis N/A:N/A (2013). PubMed: 23831006