Overview

  • Product name

    Mouse CD36 ELISA Kit
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 30 pg/ml
  • Range

    27.43 pg/ml - 20000 pg/ml
  • Recovery

    83 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 78.9 69% - 87%
    Serum 90.93 80% - 101%
    Plasma 80.53 71% - 87%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s CD36 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD36 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse CD36 coated on a 96- well plate. Standards and samples are pipetted into the wells and CD36 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse CD36 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD36 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Mouse CD36 2 vials
    CD36 Microplate (12 strips x 8 wells) 1 unit
    Recombinant Mouse CD36 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Relevance

    CD36 is a cell surface class B scavenger receptor. CD36 is preferentially found within lipid rafts, which facilitates its association with receptors, signalling and adapter molecules. CD36 binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. May function as a cell adhesion molecule. Directly mediates cyto adherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport.
  • Alternative names

    • CD36 antigen
    • CD36 antigen (collagen type I receptor thrombospondin receptor)
    • CD36 molecule
    • CD36 molecule (thrombospondin receptor)
    • CHDS7
    • Cluster determinant 36
    • FAT
    • Fatty acid translocase
    • Glycoprotein IIIb
    • GP3B
    • GP4
    • GPIIIB
    • GPIV
    • Leukocyte differentiation antigen CD36
    • PAS 4 protein
    • PAS IV
    • PASIV
    • Platelet collagen receptor
    • Platelet glycoprotein 4
    • Platelet glycoprotein IV
    • SCARB3
    • Scavenger receptor class B member 3
    • Thrombospondin receptor
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab100673

  • Representative Standard Curve using ab100673

Protocols

References

This product has been referenced in:

  • Moya-Pérez A  et al. Hepatic molecular responses to Bifidobacterium pseudocatenulatum CECT 7765 in a mouse model of diet-induced obesity. Nutr Metab Cardiovasc Dis N/A:N/A (2013). Read more (PubMed: 23831006) »
See 1 Publication for this product

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