Overview

  • Product name
    Mouse CD40 ELISA Kit
    See all CD40 kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 3 pg/ml
  • Range
    2.06 pg/ml - 1500 pg/ml
  • Recovery

    86 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 84.23 74% - 93%
    Serum 77.26 71% - 87%
    Plasma 76.68 69% - 87%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s CD40 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CD40 in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for mouse CD40 coated on a 96- well plate. Standards and samples are pipetted into the wells and CD40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get higher sensitivity in only 90 minutes with Mouse CD40 ELISA Kit (TNFRS5) (ab206307) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Mouse CD40 2 vials
    CD40 Microplate (12 x 8 wells) 1 unit
    Recombinant Mouse CD40 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    CD40 (48 to 50 kDa) is a transmembrane glycoprotein mainly expressed on the surface of B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the low affinity nerve growth factor (NGF) receptor and CD95/Fas. CD40 is the receptor for CD40 ligand. CD40 ligand (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40 predominantly on activated CD4+ T cells. Following interaction with CD40 ligand, CD40 mediates a number of major immunoregulatory functions, central to the control of thymus dependent humoral immunity and may be critical in the development of cell mediated immune responses. Other biological actions include B cell homotypic adhesion, proliferation, immunoglobulin isotype switch, and secretion. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin.
  • Alternative names
    • AI326936
    • B cell associated molecule
    • B cell associated molecule CD40
    • B cell surface antigen CD40
    • Bp50
    • CD 40
    • CD40 antigen
    • CD40 antigen (TNF receptor superfamily member 5)
    • CD40 molecule
    • CD40 molecule TNF receptor superfamily member 5
    • CD40 protein
    • CD40 type II isoform
    • CD40L receptor
    • CDw40
    • GP39
    • HIGM1
    • IGM
    • MGC9013
    • Nerve growth factor receptor related B lymphocyte activation molecule
    • NFRSF5
    • OTTHUMP00000031699
    • OTTHUMP00000031700
    • p50
    • T BAM
    • TBAM
    • TNF receptor superfamily member 5
    • TNFRSF5
    • TRAP
    • Tumor necrosis factor receptor superfamily member 5
    • Tumor necrosis factor receptor superfamily member 5 precursor
    • Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab100674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab100674

  • Representative Standard Curve using ab100674

Protocols

References

This product has been referenced in:
  • Yu H  et al. Aberrant expression of miR-663 and transforming growth factor-ß1 in nasal polyposis in children. Exp Ther Med 15:4550-4556 (2018). Read more (PubMed: 29849780) »
  • Zhao P  et al. MicroRNA-185 regulates spinal cord injuries induced by thoracolumbar spine compression fractures by targeting transforming growth factor-ß1. Exp Ther Med 13:1127-1132 (2017). ELISA ; Human . Read more (PubMed: 28450952) »
See all 2 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Abreview for CD40 Mouse ELISA Kit

Good Good 4/5 (Ease of Use)
Abreviews

Sample: Mouse Serum (Also urine samples)
Specification: Also urine samples
Amount of Sample: 50 microliter µg
Type of Kit Used: ELISA
Positive control: Provided in the kit and positive serum samples
Did you use the Abcam Kit protocol?: Yes

Abcam user community

Verified customer

Submitted Feb 12 2013

Answer

Thank you for your email.
This information is enough at the moment. I will contact you if I have any more question. Just to let you the Abreview will be processed next week because the person who is responsible for this is away at a conference.
Thank you very much for your understanding. I will get back to you soon.

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Answer

Thank you for your interest in submitting a review for ab100674.
The abreview
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Expiry date: 09-01-2013
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Please reply to this email if you have any questions.

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Answer

Thank you for your interest in Abcam's CD40 Mouse ELISA kit ab100674. Regarding a suggested dilution range for serum, we do not have any references currently available. We have not specified a range as this can vary significantly depending on the sample. We suggest testing multiple dilutions in a trial run to determine which dilution will be most suitable for your samples. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions. Best of luck with your experiments!

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