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    mouse-cd40l-elisa-kit-ab119517.pdf

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Immunology Adaptive Immunity T Cells CD
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Mouse CD40L ELISA Kit (ab119517)

  • Datasheet
  • SDS
  • Protocol Booklet
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Typical Standard Curve

    Key features and details

    • Sensitivity: 0.14 ng/ml
    • Range: 0.31 ng/ml - 20 ng/ml
    • Sample type: Cell culture supernatant, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Mouse

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    Recombinant mouse TRAP/CD40L protein (Soluble) (ab50160)

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    Overview

    • Product name

      Mouse CD40L ELISA Kit
      See all TRAP/CD40L kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall 8 6.5%
      Inter-assay
      Sample n Mean SD CV%
      Overall 8 11.1%
    • Sample type

      Cell culture supernatant, Serum
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      0.14 ng/ml
    • Range

      0.31 ng/ml - 20 ng/ml
    • Recovery

      94 %

    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Mouse
    • Product overview

      Abcam’s CD40L Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Mouse CD40L concentrations in cell culture supernatant and serum.

      CD40L specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated CD40L detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue product that changes to yellow after the addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of CD40L captured on the plate.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 1 x 96 tests
      20X Assay Buffer Concentrate 1 x 5ml
      20X Wash Buffer Concentrate 1 x 50ml
      Adhesive Films 4 units
      Biotin-Conjugate anti-mouse CD40L monoclonal antibody 1 x 70µl
      Microplate coated with monoclonal antibody to mouse CD40L (12 x 8 wells) 1 unit
      Mouse CD40L Standard lyophilized (40 ng /mL upon reconstitution) 2 vials
      Sample Diluent 1 x 12ml
      Stop Solution (1M Phosphoric acid) 1 x 15ml
      Streptavidin-HRP 1 x 150µl
      TMB Substrate Solution 1 x 15ml
    • Research areas

      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Immunology
      • Immunoglobulins
      • Class Switching
      • Immunology
      • Innate Immunity
      • Cytokines
      • TNF Superfamily
      • Cardiovascular
      • Atherosclerosis
      • Thrombosis
      • Platelets
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Innate and adaptive immunity
      • Immunology
      • Adaptive Immunity
      • Regulatory T Cells
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • CD markers ELISA kits
    • Function

      Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
      Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
    • Tissue specificity

      Specifically expressed on activated CD4+ T-lymphocytes.
    • Involvement in disease

      Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
    • Sequence similarities

      Belongs to the tumor necrosis factor family.
    • Post-translational
      modifications

      The soluble form derives from the membrane form by proteolytic processing.
      N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
      Not O-glycosylated.
    • Cellular localization

      Secreted and Cell membrane.
    • Target information above from: UniProt accession P29965 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • CD 40L
      • CD154
      • CD40 antigen ligand
      • CD40 ligand
      • CD40 ligand, soluble form
      • CD40-L
      • CD40L
      • CD40L_HUMAN
      • CD40LG
      • gp39
      • hCD40L
      • HIGM1
      • IGM
      • IMD3
      • T B cell activating molecule
      • T BAM
      • T-cell antigen Gp39
      • TNF-related activation protein
      • TNFSF5
      • TrAP
      • Tumor necrosis factor (ligand) superfamily member 5
      • Tumor necrosis factor ligand superfamily member 5
      see all
    • Database links

      • Entrez Gene: 21947 Mouse
      • SwissProt: P27548 Mouse
      • Unigene: 4861 Mouse

      Associated products

        Images

        • Typical Standard Curve
          Typical Standard Curve

          Representative Standard Curve using ab119517.

        Protocols

        • Protocol Booklet

        Click here to view the general protocols

        Datasheets and documents

        • Datasheet
        • SDS
      • References (1)

        Publishing research using ab119517? Please let us know so that we can cite the reference in this datasheet.

        ab119517 has been referenced in 1 publication.

        • Thomas S  et al. Development of a new fusion-enhanced oncolytic immunotherapy platform based on herpes simplex virus type 1. J Immunother Cancer 7:214 (2019). PubMed: 31399043

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