Overview

  • Product name

    Mouse CD8 alpha ELISA Kit
    See all CD8 alpha kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Mouse thymus 8 8.8%
    Inter-assay
    Sample n Mean SD CV%
    Mouse thymus 3 5.3%
  • Sample type

    Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    46 pg/ml
  • Range

    0.31 ng/ml - 20 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Tissue Extracts 104 91% - 129%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    CD8 alpha in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD8 alpha protein in mouse tissue extract.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Mouse CD8 alpha, or T-Cell surface glycoprotein CD8 alpha chain, is a cell surface glycoprotein encoded by the gene Cd8a. CD8 alpha is found on cytotoxic T lymphocytes and helps mediates cell-cell interactions within the immune system. CD8 acts a coreceptor for MHC class 1/peptide complex either as a homodimer of two alpha chains or as a heterodimer of one alpha chain and one beta chain. CD8 alpha interacts with LCK in a zinc-dependent manner, which initiates intracellular signaling pathways resulting in activation of cytotoxic T-lymphocytes.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse CD8 alpha Capture Antibody 1 x 600µl
    10X Mouse CD8 alpha Detector Antibody 1 x 600µl
    Mouse CD8 alpha Lyophilized Recombinant Protein 2 vials
    Antibody Diluent CPR 1 x 6ml
    10X Wash Buffer PT (ab206977) 1 x 20ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    50X Cell Extraction Enhancer Solution 1 x 1ml
    TMB Development Solution 1 x 12ml
    Stop Solution 1 x 12ml
    Sample Diluent NS 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Plate Seals 1 unit
  • Research areas

  • Function

    Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in disease

    Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Alternative names

    • alpha polypeptide (p32)
    • CD8
    • CD8 antigen alpha polypeptide
    • CD8 antigen alpha polypeptide (p32)
    • CD8a
    • CD8A antigen
    • CD8A molecule
    • CD8A_HUMAN
    • Leu2
    • Leu2 T lymphocyte antigen
    • Ly3
    • LYT3
    • MAL
    • OKT8 T cell antigen
    • OTTHUMP00000160760
    • OTTHUMP00000160764
    • OTTHUMP00000203528
    • OTTHUMP00000203721
    • p32
    • T cell antigen Leu2
    • T cell co receptor
    • T-cell surface glycoprotein CD8 alpha chain
    • T-lymphocyte differentiation antigen T8/Leu-2
    • T8 T cell antigen
    • T8/Leu-2 T-lymphocyte differentiation antigen
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab238264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of CD8 alpha were measured in duplicates, interpolated from the CD8 alpha standard curves and corrected for sample dilution. Undiluted samples are as follows: thymus 400 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Protocols

References

ab238264 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

CD8 ELISA

Average Excellent 5/5 (Ease of Use)
Abreviews
The standards are good. Doesn't work with cell culture supernatant. Tried for BV-2 mouse microglia cell line.

Miss. Sheelu Monga

Verified customer

Submitted Aug 28 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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