Overview

  • Product name

    Mouse CD93 ELISA Kit
    See all CD93 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 4.2%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 5.7%
  • Sample type

    Serum, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    3.4 pg/ml
  • Range

    23.4 pg/ml - 1500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 102 98% - 107%
    Tissue Extracts 99 97% - 112%
    Hep Plasma 108 101% - 113%
    EDTA Plasma 101 98% - 106%
    Cit plasma 103 99% - 110%
    Tissue Homogenate 108 102% - 117%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Cow
  • Product overview

    CD93 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD93 protein in mouse serum, plasma, tissue supernatant, and tissue homogenate extract samples


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    CD93 is a transmembrane glycoprotein receptor for C1q, MBL2, and SPA. It is involved in the immune response and in hematopoietic and vascular development. Elevated CD93 levels are seen during peritonitis, sepsis, and rheumatoid arthritis. Altered CD93 serum levels correlates with a decreased risk of coronary artery disease and myocardial infarction.


    Sensitivity:


    Samples diluted in Sample Diluent NS - 10.4 pg/mL


    Samples diluted in 1X Cell Extraction Buffer PTR - 3.4 pg/mL

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse CD93 Capture Antibody 1 x 600µl
    10X Mouse CD93 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BR 1 x 6ml
    Mouse CD93 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Receptor (or element of a larger receptor complex) for C1q, mannose-binding lectin (MBL2) and pulmonary surfactant protein A (SPA). May mediate the enhancement of phagocytosis in monocytes and macrophages upon interaction with soluble defense collagens. May play a role in intercellular adhesion.
  • Tissue specificity

    Highly expressed in endothelial cells, platelets, cells of myeloid origin, such as monocytes and neutrophils. Not expressed in cells of lymphoid origin.
  • Sequence similarities

    Contains 1 C-type lectin domain.
    Contains 5 EGF-like domains.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Alternative names

    • C1q receptor 1
    • C1q/MBL/SPA receptor
    • C1qR
    • C1qR(p)
    • C1qr1
    • C1QR1_HUMAN
    • C1qRp
    • CD93
    • CD93 antigen
    • CD93 molecule
    • CDw93
    • Complement component 1 q subcomponent receptor 1
    • Complement component C1q receptor
    • dJ737E23.1
    • ECSM3
    • Matrix remodeling associated protein 4
    • Matrix remodelling associated 4
    • Matrix-remodeling-associated protein 4
    • MXRA4
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab233620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard curve comparison between Mouse CD93 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of CD93 were measured in duplicates, interpolated from the CD93 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1: 1,000, plasma (citrate) 1: 500, plasma (heparin) 1: 1,000, and plasma (EDTA) 1: 500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD93 concentration was determined to be 863 ng/mL in serum, 633 ng/mL in plasma (citrate), 799 ng/ml in plasma (heparin), and 468 ng/ml in plasma (EDTA).

  • The concentrations of CD93 were measured in duplicates, interpolated from the CD93 standard curves and corrected for sample dilution. Undiluted samples are as follows: mouse lung supernatant 1: 200 and mouse heart supernatant 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD93 concentration was determined to be 103 ng/mL in mouse lung supernatant and 50 ng/ml in mouse heart supernatant.

  • The concentrations of CD93 were measured in duplicate and interpolated from the CD93 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD93 concentration was determined to be 898 pg/mL in lung homogenate extract, 1116 pg/ml in heart homogenate extract, and 1133 ng/mL in spleen homogenate extract samples.

Protocols

References

ab233620 has not yet been referenced specifically in any publications.

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